CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000676
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glycosylphosphatidylinositol anchor attachment 1 protein 
Protein Synonyms/Alias
 GPI anchor attachment protein 1; GAA1 protein homolog; hGAA1 
Gene Name
 GPAA1 
Gene Synonyms/Alias
 GAA1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
82RDFAAHRKKSGALPVubiquitination[1]
83DFAAHRKKSGALPVAubiquitination[1, 2]
111YTQSFSRKLPFPDETubiquitination[1, 2, 3]
329INSFRQYKYDLVAVGubiquitination[2, 4, 5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate. 
Sequence Annotation
 CARBOHYD 203 203 N-linked (GlcNAc...) (Potential).
 CARBOHYD 517 517 N-linked (GlcNAc...) (Potential).  
Keyword
 Alternative splicing; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; GPI-anchor biosynthesis; Membrane; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 621 AA 
Protein Sequence
MGLLSDPVRR RALARLVLRL NAPLCVLSYV AGIAWFLALV FPPLTQRTYM SENAMGSTMV 60
EEQFAGGDRA RAFARDFAAH RKKSGALPVA WLERTMRSVG LEVYTQSFSR KLPFPDETHE 120
RYMVSGTNVY GILRAPRAAS TESLVLTVPC GSDSTNSQAV GLLLALAAHF RGQIYWAKDI 180
VFLVTEHDLL GTEAWLEAYH DVNVTGMQSS PLQGRAGAIQ AAVALELSSD VVTSLDVAVE 240
GLNGQLPNLD LLNLFQTFCQ KGGLLCTLQG KLQPEDWTSL DGPLQGLQTL LLMVLRQASG 300
RPHGSHGLFL RYRVEALTLR GINSFRQYKY DLVAVGKALE GMFRKLNHLL ERLHQSFFLY 360
LLPGLSRFVS IGLYMPAVGF LLLVLGLKAL ELWMQLHEAG MGLEEPGGAP GPSVPLPPSQ 420
GVGLASLVAP LLISQAMGLA LYVLPVLGQH VATQHFPVAE AEAVVLTLLA IYAAGLALPH 480
NTHRVVSTQA PDRGWMALKL VALIYLALQL GCIALTNFSL GFLLATTMVP TAALAKPHGP 540
RTLYAALLVL TSPAATLLGS LFLWRELQEA PLSLAEGWQL FLAALAQGVL EHHTYGALLF 600
PLLSLGLYPC WLLFWNVLFW K 621 
Gene Ontology
 GO:0042765; C:GPI-anchor transamidase complex; TAS:UniProtKB.
 GO:0015631; F:tubulin binding; NAS:UniProtKB.
 GO:0016255; P:attachment of GPI anchor to protein; NAS:UniProtKB.
 GO:0006501; P:C-terminal protein lipidation; TAS:Reactome.
 GO:0006461; P:protein complex assembly; NAS:UniProtKB.
 GO:0006621; P:protein retention in ER lumen; NAS:UniProtKB. 
Interpro
 IPR007246; Gaa1.
 IPR017063; GPI_prot_transamidse_cplx_GAA1. 
Pfam
 PF04114; Gaa1 
SMART
  
PROSITE
  
PRINTS