CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023131
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 BAG family molecular chaperone regulator 5 
Protein Synonyms/Alias
 BAG-5; Bcl-2-associated athanogene 5 
Gene Name
 BAG5 
Gene Synonyms/Alias
 KIAA0873 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
251EVVEDINKLLKYLDLubiquitination[1]
254EDINKLLKYLDLEEEubiquitination[1]
278RQNHSILKIEKVLKRacetylation[2, 3]
356KEALEKRKLFACEEHubiquitination[1]
404RLEELLTKQLLALDAubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Inhibits both auto-ubiquitination of PARK2 and ubiquitination of target proteins by PARK2 (By similarity). May function as a nucleotide exchange factor for HSP/HSP70, promoting ADP release, and activating Hsp70-mediated refolding. 
Sequence Annotation
 DOMAIN 9 86 BAG 1.
 DOMAIN 95 167 BAG 2.
 DOMAIN 182 260 BAG 3.
 DOMAIN 275 350 BAG 4.
 DOMAIN 365 442 BAG 5.  
Keyword
 3D-structure; Alternative splicing; Chaperone; Complete proteome; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 447 AA 
Protein Sequence
MDMGNQHPSI SRLQEIQKEV KSVEQQVIGF SGLSDDKNYK KLERILTKQL FEIDSVDTEG 60
KGDIQQARKR AAQETERLLK ELEQNANHPH RIEIQNIFEE AQSLVREKIV PFYNGGNCVT 120
DEFEEGIQDI ILRLTHVKTG GKISLRKARY HTLTKICAVQ EIIEDCMKKQ PSLPLSEDAH 180
PSVAKINFVM CEVNKARGVL IALLMGVNNN ETCRHLSCVL SGLIADLDAL DVCGRTEIRN 240
YRREVVEDIN KLLKYLDLEE EADTTKAFDL RQNHSILKIE KVLKRMREIK NELLQAQNPS 300
ELYLSSKTEL QGLIGQLDEV SLEKNPCIRE ARRRAVIEVQ TLITYIDLKE ALEKRKLFAC 360
EEHPSHKAVW NVLGNLSEIQ GEVLSFDGNR TDKNYIRLEE LLTKQLLALD AVDPQGEEKC 420
KAARKQAVRL AQNILSYLDL KSDEWEY 447 
Gene Ontology
 GO:0016234; C:inclusion body; IDA:BHF-UCL.
 GO:0048471; C:perinuclear region of cytoplasm; IDA:BHF-UCL.
 GO:0031625; F:ubiquitin protein ligase binding; ISS:BHF-UCL.
 GO:0061084; P:negative regulation of protein refolding; ISS:BHF-UCL.
 GO:0051444; P:negative regulation of ubiquitin-protein ligase activity; ISS:BHF-UCL.
 GO:0070997; P:neuron death; ISS:BHF-UCL.
 GO:0006457; P:protein folding; TAS:ProtInc.
 GO:0090083; P:regulation of inclusion body assembly; IMP:BHF-UCL. 
Interpro
 IPR003103; BAG_domain. 
Pfam
 PF02179; BAG 
SMART
 SM00264; BAG 
PROSITE
 PS51035; BAG 
PRINTS