CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006260
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitosis inhibitor protein kinase SWE1 
Protein Synonyms/Alias
 Wee1 homolog 
Gene Name
 SWE1 
Gene Synonyms/Alias
 YJL187C; J0406 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
594ITFEGNLKLGDFGMAsumoylation[1]
741NVNTAATKNRLILHKubiquitination[2, 3]
Reference
 [1] Cdk1 and SUMO regulate Swe1 stability.
 Simpson-Lavy KJ, Brandeis M.
 PLoS One. 2010 Dec 6;5(12):e15089. [PMID: 21151918]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Protein kinase that acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylating and inhibiting the mitosis-promoting cyclin B-bound CDC28 at 'Tyr-19'. SWE1-mediated inhibition of CDC28 acts in a cell size or morphogenesis checkpoint to delay mitosis in response to defects in growth, actin organization or bud formation. Inhibits the activity of B-type cyclins in replication initiation strongly for CLB2, moderately for CLB3 and CLB4, and there is no apparent inhibition for CLB5 and CLB6, correlating with the normal expression timing of those cyclins. Hyperphosphorylation and degradation of SWE1 when all checkpoint requirement are met releases CLB2-CDC28 from inhibition and allows for progression through the cell cycle. SWE1-dependent CDC28 phosphorylation is also required for pachytene arrest upon activation of the recombination checkpoint during meiosis. Also involved in the regulation of nitrogen starvation- and short chain alcohol-induced filamentous growth, or filamentous differentiation in response to slowed DNA synthesis. Can act both on serines and on tyrosines. 
Sequence Annotation
 DOMAIN 444 794 Protein kinase.
 NP_BIND 450 458 ATP (By similarity).
 ACT_SITE 579 579 Proton acceptor (By similarity).
 METAL 584 584 Magnesium; via carbonyl oxygen (By
 METAL 597 597 Magnesium; via carbonyl oxygen (By
 BINDING 473 473 ATP (By similarity).
 MOD_RES 36 36 Phosphoserine; by CDC5.
 MOD_RES 45 45 Phosphothreonine; by CDC28.
 MOD_RES 56 56 Phosphoserine; by CDC28.
 MOD_RES 63 63 Phosphoserine; by CDC28.
 MOD_RES 70 70 Phosphoserine.
 MOD_RES 74 74 Phosphothreonine; by CDC28.
 MOD_RES 102 102 Phosphoserine; by CDC5.
 MOD_RES 105 105 Phosphoserine; by CDC28.
 MOD_RES 111 111 Phosphoserine; by CDC5, CDC28 and CLA4.
 MOD_RES 118 118 Phosphoserine; by CDC5.
 MOD_RES 121 121 Phosphothreonine; by CDC28.
 MOD_RES 124 124 Phosphothreonine; by CDC28.
 MOD_RES 127 127 Phosphoserine; by CDC28.
 MOD_RES 131 131 Phosphothreonine; by CDC5.
 MOD_RES 133 133 Phosphoserine; by CDC28.
 MOD_RES 136 136 Phosphoserine; by CDC28 and CLA4.
 MOD_RES 156 156 Phosphoserine; by CDC5.
 MOD_RES 169 169 Phosphoserine; by CDC5.
 MOD_RES 196 196 Phosphothreonine; by CDC28.
 MOD_RES 201 201 Phosphoserine; by CDC28.
 MOD_RES 225 225 Phosphoserine; by CDC5.
 MOD_RES 254 254 Phosphoserine; by CDC5.
 MOD_RES 262 262 Phosphoserine.
 MOD_RES 263 263 Phosphoserine; by CDC28.
 MOD_RES 266 266 Phosphoserine; by CDC28.
 MOD_RES 280 280 Phosphothreonine; by CDC5.
 MOD_RES 284 284 Phosphoserine.
 MOD_RES 294 294 Phosphoserine.
 MOD_RES 312 312 Phosphoserine; by CLA4.
 MOD_RES 345 345 Phosphoserine.
 MOD_RES 367 367 Phosphothreonine; by CDC28.
 MOD_RES 373 373 Phosphothreonine; by CDC28.
 MOD_RES 379 379 Phosphoserine; by CDC5 and CLA4.
 MOD_RES 384 384 Phosphothreonine; by CDC28.
 MOD_RES 395 395 Phosphoserine; by CDC5 and CLA4.
 MOD_RES 438 438 Phosphoserine; by CDC5 and CLA4.
 MOD_RES 610 610 Phosphoserine; by CDC5.
 MOD_RES 629 629 Phosphothreonine; by CDC5.
 MOD_RES 688 688 Phosphothreonine; by CDC5 and CLA4.
 MOD_RES 692 692 Phosphothreonine.  
Keyword
 ATP-binding; Cell cycle; Cell division; Complete proteome; Kinase; Magnesium; Meiosis; Metal-binding; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase; Tyrosine-protein kinase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 819 AA 
Protein Sequence
MSSLDEDEED FEMLDTENLQ FMGKKMFGKQ AGEDESDDFA IGGSTPTNKL KFYPYSNNKL 60
TRSTGTLNLS LSNTALSEAN SKFLGKIEEE EEEEEEGKDE ESVDSRIKRW SPFHENESVT 120
TPITKRSAEK TNSPISLKQW NQRWFPKNDA RTENTSSSSS YSVAKPNQSA FTSSGLVSKM 180
SMDTSLYPAK LRIPETPVKK SPLVEGRDHK HVHLSSSKNA SSSLSVSPLN FVEDNNLQED 240
LLFSDSPSSK ALPSIHVPTI DSSPLSEAKY HAHDRHNNQT NILSPTNSLV TNSSPQTLHS 300
NKFKKIKRAR NSVILKNREL TNSLQQFKDD LYGTDENFPP PIIISSHHST RKNPQPYQFR 360
GRYDNDTDEE ISTPTRRKSI IGATSQTHRE SRPLSLSSAI VTNTTSAETH SISSTDSSPL 420
NSKRRLISSN KLSANPDSHL FEKFTNVHSI GKGQFSTVYQ VTFAQTNKKY AIKAIKPNKY 480
NSLKRILLEI KILNEVTNQI TMDQEGKEYI IDYISSWKFQ NSYYIMTELC ENGNLDGFLQ 540
EQVIAKKKRL EDWRIWKIIV ELSLALRFIH DSCHIVHLDL KPANVMITFE GNLKLGDFGM 600
ATHLPLEDKS FENEGDREYI APEIISDCTY DYKADIFSLG LMIVEIAANV VLPDNGNAWH 660
KLRSGDLSDA GRLSSTDIHS ESLFSDITKV DTNDLFDFER DNISGNSNNA GTSTVHNNSN 720
INNPNMNNGN DNNNVNTAAT KNRLILHKSS KIPAWVPKFL IDGESLERIV RWMIEPNYER 780
RPTANQILQT EECLYVEMTR NAGAIIQEDD FGPKPKFFI 819 
Gene Ontology
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0004713; F:protein tyrosine kinase activity; IDA:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000078; P:cell shape checkpoint; IDA:SGD.
 GO:0000086; P:G2/M transition of mitotic cell cycle; IMP:SGD.
 GO:0007126; P:meiosis; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0010697; P:negative regulation of spindle pole body separation; IMP:SGD.
 GO:0000320; P:re-entry into mitotic cell cycle; IGI:SGD.
 GO:0008361; P:regulation of cell size; IMP:SGD.
 GO:0000079; P:regulation of cyclin-dependent protein serine/threonine kinase activity; IDA:SGD.
 GO:0040020; P:regulation of meiosis; IGI:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS