UniProt Accession

 NAA20_HUMAN; P61599; A6NHA3; B2R4G4; Q5TFT7; Q9D7H8; Q9H0Y4; Q9NQH6; Q9Y6D2 

Genbank Protein ID

 AF085355; AL136641; AK311819; AL049538; AL035454; AL035454; AL049538; AL035454; AL049538; AL049538; AL035454; CH471133; CH471133; BC005181; BC008446; BG548527 

Genbank Nucleotide ID

 AAD40190.1; CAB66576.1; BAG34761.1; CAI19341.1; CAI19341.1; CAI42118.1; CAI42118.1; CAX15127.1; CAX15127.1; CAX15212.1; CAX15212.1; EAX10214.1; EAX10215.1; AAH05181.1; AAH08446.1 

Protein Name

 N-alpha-acetyltransferase 20 

Protein Synonyms/Alias

 Methionine N-acetyltransferase; N-acetyltransferase 5; N-terminal acetyltransferase B complex catalytic subunit NAA20; N-terminal acetyltransferase B complex catalytic subunit NAT5; NatB complex subunit NAT5; NatB catalytic subunit 

Gene Name

 NAA20 

Gene Synonyms/Alias

 NAT5 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
103	LEEISERKGGFFVDL	ubiquitination	[1]
124	QVAVNMYKQLGYSVY	ubiquitination	[1, 2, 3, 4, 5]
163	LSRDTEKKSIIPLPH	ubiquitination	[1, 3, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp, Met-Glu, Met-Asn and Met-Gln. Proteins with cell cycle functions are overrepresented in the pool of NatB substrates. Required for maintaining the structure and function of actomyosin fibers and for proper cellular migration. 

Sequence Annotation

 DOMAIN 2 157 N-acetyltransferase.  

Keyword

 Acyltransferase; Alternative splicing; Complete proteome; Cytoplasm; Nucleus; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 178 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005622; C:intracellular; IDA:LIFEdb.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0004596; F:peptide alpha-N-acetyltransferase activity; IEA:EC. 

Interpro

 IPR016181; Acyl_CoA_acyltransferase.
 IPR000182; GNAT_dom. 

Pfam

 PF00583; Acetyltransf_1 

SMART

  

PROSITE

 PS51186; GNAT 

PRINTS