UniProt Accession

 CSN2_HUMAN; P61201; O88950; Q15647; Q6FGP4; Q9BY54; Q9R249; Q9UNI2; Q9UNQ5 

Genbank Protein ID

 AF084260; AF100762; AF212227; CR542063; BC012629; L40388; AF120268 

Genbank Nucleotide ID

 AAC34122.1; AAD43026.1; AAK26250.1; CAG46860.1; AAH12629.1; AAC41734.1; AAD30269.1 

Protein Name

 COP9 signalosome complex subunit 2 

Protein Synonyms/Alias

 SGN2; Signalosome subunit 2; Alien homolog; JAB1-containing signalosome subunit 2; Thyroid receptor-interacting protein 15; TR-interacting protein 15; TRIP-15 

Gene Name

 COPS2 

Gene Synonyms/Alias

 CSN2; TRIP15 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
232	YEQSLHIKSAIPHPL	ubiquitination	[1]
338	TEFEKILKTNHSNIM	ubiquitination	[2]
371	QVLIKLIKPYTRIHI	ubiquitination	[1]
422	LLELDHQKRGGARYT	ubiquitination	[1, 2, 3, 4]
433	ARYTALDKWTNQLNS	ubiquitination	[1, 2, 3, 4]
448	LNQAVVSKLA*****	ubiquitination	[1, 2, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN- dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1. 

Sequence Annotation

 DOMAIN 248 413 PCI.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Direct protein sequencing; Nucleus; Phosphoprotein; Reference proteome; Signalosome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 443 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0008180; C:signalosome; IDA:UniProtKB.
 GO:0004871; F:signal transducer activity; NAS:UniProtKB.
 GO:0003714; F:transcription corepressor activity; IEA:Compara.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0010388; P:cullin deneddylation; IDA:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0030182; P:neuron differentiation; IEA:Compara.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:UniProtKB. 

Interpro

 IPR013143; PAM.
 IPR000717; PCI_dom.
 IPR011990; TPR-like_helical.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF01399; PCI 

SMART

 SM00753; PAM
 SM00088; PINT 

PROSITE

  

PRINTS