CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030810
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Translation initiation factor eIF-2B subunit alpha 
Protein Synonyms/Alias
 cDNA FLJ60506, highly similar to Translation initiation factor eIF-2B subunit alpha 
Gene Name
 EIF2B1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15EYFKSQMKEDPDMASubiquitination[1, 2]
35RTLLEFLKRDKGETIubiquitination[3]
38LEFLKRDKGETIQGLubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Initiation factor; Protein biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 222 AA 
Protein Sequence
MDDKELIEYF KSQMKEDPDM ASAVAAIRTL LEFLKRDKGE TIQGLRANLT SAIETLCGVD 60
SSVAVSSGGE LFLRFISLAS LEYSDYSKCK KIMIERGELF LRRISLSRNK IADLCHTFIK 120
DGATILTHAY SRVVLRVLEA AVAAKKRFSV YVTESQPDLS GQVPFCSVMC PAIILQSKLR 180
ITVQQDQNQN VPPACQQSAL PFIVPFPAFG RKITEFAAGR SI 222 
Gene Ontology
 GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW. 
Interpro
 IPR000649; IF-2B-related. 
Pfam
 PF01008; IF-2B 
SMART
  
PROSITE
  
PRINTS