CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016016
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Nischarin 
Protein Synonyms/Alias
 Imidazoline receptor 1; I-1; IR1; Imidazoline receptor I-1-like protein; Imidazoline-1 receptor; I1R 
Gene Name
 Nisch 
Gene Synonyms/Alias
 Kiaa0975 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
425IIPDYRTKVLSQFGEubiquitination[1]
865VYLVYSDKRMVQTPAubiquitination[1]
1009RASLQDLKTVVISKNubiquitination[1]
1379PSPEPVDKDFYSEFGubiquitination[1]
1388FYSEFGDKNTGKMENubiquitination[1]
1392FGDKNTGKMENYELIubiquitination[1]
1405LIHSSRVKFTYPSEEubiquitination[1]
1570PSAESREKLISLLARubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1- stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis (By similarity). Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3 (By similarity). When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures (By similarity). 
Sequence Annotation
 DOMAIN 12 122 PX.
 REPEAT 290 311 LRR 1.
 REPEAT 313 334 LRR 2.
 REPEAT 335 356 LRR 3.
 REPEAT 358 379 LRR 4.
 REPEAT 380 401 LRR 5.
 REPEAT 405 426 LRR 6.
 REPEAT 1081 1086 1.
 REPEAT 1087 1092 2.
 REPEAT 1093 1098 3.
 REPEAT 1099 1104 4.
 REPEAT 1105 1110 5.
 REPEAT 1111 1116 6.
 REPEAT 1123 1128 7.
 REPEAT 1129 1134 8.
 REPEAT 1135 1140 9.
 REPEAT 1141 1146 10.
 REGION 1 134 Necessary for binding to
 REGION 121 695 Necessary for homooligomerization and
 REGION 246 869 Interaction with PAK1.
 REGION 661 869 Interaction with LIMK.
 REGION 709 807 Interaction with ITGA5.
 REGION 1081 1146 10 X 6 AA tandem repeat of A-E-A-P-A-A.
 MOD_RES 1373 1373 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Apoptosis; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Endosome; Leucine-rich repeat; Membrane; Phosphoprotein; Receptor; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1593 AA 
Protein Sequence
MAAATLSFGP EREAEPAKEA RVVGSELVDT YTVYVIQVTD GNHEWTIKHR YSDFHDLHEK 60
LVAERKIDKS LLPPKKIIGK NSRSLVEKRE RDLEVYLQTL LTTFPDVAPR VLAHFLHFHL 120
YEVNGVTAAL AEELFEKGEQ LLGAGEVFAI RPLQLYAITE QLQQGKPTCA SGDAKTDLGH 180
ILDFTCRLKY LKVSGTEGPF GTSNIKEQLL PFDLSIFKSL HQVEISHCDA KHIRGLVTSK 240
PTLATMSVRF SATSMKEVLA PEASEFDEWE PEGTATLGGP VTAIIPTWQA LTTLDLSHNS 300
ICEIDESVKL IPKIEYLDLS HNGLRVVDNL QHLYNLVHLD LSYNKLSSLE GVHTKLGNVK 360
TLNLAGNFLE SLSGLHKLYS LVNVDLRDNR IEQLDEVKSI GSLPCLERLT LLNNPLSIIP 420
DYRTKVLSQF GERASEICLD DVATTEKELD TVEVLKAIQK AKDVKSKLSN TEKKAGEDFR 480
LPPAPCIRPG GSPPAAPASA SLPQPILSNQ GIMFVQEEAL ASSLSSTDSL PPEDHRPIAR 540
ACSDSLESIP AGQVASDDLR DVPGAVGGVS PDHAEPEVQV VPGSGQIIFL PFTCIGYTAT 600
NQDFIQRLST LIRQAIERQL PAWIEAANQR EEAHGEQGEE EEEEEEEEDV AENRYFEMGP 660
PDAEEEEGSG QGEEDEEDED EEAEEERLAL EWALGADEDF LLEHIRILKV LWCFLIHVQG 720
SIRQFAACLV LTDFGIAVFE IPHQESRGSS QHILSSLRFV FCFPHGDLTE FGFLMPELCL 780
VLKVRHSENT LFIISDAANL HEFHADLRSC FAPQHMAMLC SPILYGSHTT LQEFLRQLLT 840
FYKVAGGSQE RSQGCFPVYL VYSDKRMVQT PAGDYSGNIE WASCTLCSAV RRSCCAPSEA 900
VKSAAIPYWL LLTSQHLNVI KADFNPMPNR GTHNCRNRNS FKLSRVPLST VLLDPTRSCT 960
QPRGAFADGH VLELLVGYRF VTAIFVLPHE KFHFLRVYNQ LRASLQDLKT VVISKNPSAK 1020
PRNQPAKSRA SAEQRLQETP ADAPAPAAVP PTASAPAPAE ALAPDLAPVQ APGEDRGLTS 1080
AEAPAAAEAP AAAEAPAAAE APAAAEAPAA AEAPAAAEAP APAEAPAAAE APAAAEAPAA 1140
AEAPAAAEAP ASAEAPAPNQ APAPARGPAP ARGPAPAGGP APAEALAQAE VPAQYPSERL 1200
IQSTSEENQI PSHLPVCPSL QHIARLRGRA IIDLFHNSIA EVENEELRHL LWSSVVFYQT 1260
PGLEVTACVL LSSKAVYFIL HDGLRRYFSE PLQDFWHQKN TDYNNSPFHV SQCFVLKLSD 1320
LQSVNVGLFD QYFRLTGSSP TQVVTCLTRD SYLTHCFLQH LMLVLSSLER TPSPEPVDKD 1380
FYSEFGDKNT GKMENYELIH SSRVKFTYPS EEEVGDLTYI VAQKMADPAK NPALSILLYI 1440
QAFQVVTPHL GRGRGPLRPK TLLLTSAEIF LLDEDYIHYP LPEFAKEPPQ RDRYRLDDGR 1500
RVRDLDRVLM GYYPYPQALT LVFDDTQGHD LMGSVTLDHF GEMPGGPGRV GQGREVQWQV 1560
FVPSAESREK LISLLARQWE ALCGRELPVE LTG 1593 
Gene Ontology
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0005769; C:early endosome; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0055037; C:recycling endosome; IEA:UniProtKB-SubCell.
 GO:0008227; F:G-protein coupled amine receptor activity; IEA:Compara.
 GO:0005178; F:integrin binding; IDA:MGI.
 GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
 GO:0030036; P:actin cytoskeleton organization; IDA:MGI.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0006006; P:glucose metabolic process; IEA:Compara.
 GO:0030336; P:negative regulation of cell migration; IDA:MGI.
 GO:0048243; P:norepinephrine secretion; IEA:Compara.
 GO:0016601; P:Rac protein signal transduction; IDA:MGI.
 GO:0008217; P:regulation of blood pressure; IEA:Compara.
 GO:0032228; P:regulation of synaptic transmission, GABAergic; IEA:Compara. 
Interpro
 IPR001611; Leu-rich_rpt.
 IPR001683; Phox. 
Pfam
 PF00787; PX 
SMART
 SM00312; PX 
PROSITE
 PS51450; LRR
 PS50195; PX 
PRINTS