CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030642
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase 24 
Protein Synonyms/Alias
 cDNA FLJ16404 fis, clone UTERU2008019, highly similar to Serine/threonine-protein kinase 3 (EC 2.7.11.1) 
Gene Name
 STK3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
49LSEDSLTKQPEEVFDubiquitination[1]
84SGQVVAIKQVPVESDubiquitination[1, 2]
144DIIRLRNKTLIEDEIubiquitination[2, 3]
176RKIHRDIKAGNILLNubiquitination[2, 3, 4]
205QLTDTMAKRNTVIGTubiquitination[1, 2, 3, 4, 5]
271NPPPTFRKPELWSDDubiquitination[2, 3, 5]
284DDFTDFVKKCLVKNPubiquitination[1]
326ITEAMEIKAKRHEEQubiquitination[1, 2]
481EELQMRLKALDPMMEubiquitination[3]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 519 AA 
Protein Sequence
MLQLMDSGIT ICLRNGAASV FKKKEWSTQG EENKQDSKLK KLSEDSLTKQ PEEVFDVLEK 60
LGEGSYGSVF KAIHKESGQV VAIKQVPVES DLQEIIKEIS IMQQCDSPYV VKYYGSYFKN 120
TDLWIVMEYC GAGSVSDIIR LRNKTLIEDE IATILKSTLK GLEYLHFMRK IHRDIKAGNI 180
LLNTEGHAKL ADFGVAGQLT DTMAKRNTVI GTPFWMAPEV IQEIGYNCVA DIWSLGITSI 240
EMAEGKPPYA DIHPMRAIFM IPTNPPPTFR KPELWSDDFT DFVKKCLVKN PEQRATATQL 300
LQHPFIKNAK PVSILRDLIT EAMEIKAKRH EEQQRELEEE EENSDEDELD SHTMVKTSVE 360
SVGTMRATST MSEGAQTMIE HNSTMLESDL GTMVINSEDE EEEDGTMKRN ATSPQVQRPS 420
FMDYFDKQDF KNKSHENCNQ NMHEPFPMSK NVFPDNWKVP QDGDFDFLKN LSLEELQMRL 480
KALDPMMERE IEELRQRYTA KRQPILDAMD AKKRRQQNF 519 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
 GO:0060706; P:cell differentiation involved in embryonic placenta development; IEA:Compara.
 GO:0007417; P:central nervous system development; IEA:Compara.
 GO:0003157; P:endocardium development; IEA:Compara.
 GO:0035329; P:hippo signaling cascade; IEA:Compara.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0046621; P:negative regulation of organ growth; IEA:Compara.
 GO:0001841; P:neural tube formation; IEA:Compara.
 GO:0046330; P:positive regulation of JNK cascade; IEA:Compara.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0060215; P:primitive hemopoiesis; IEA:Compara. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR024205; Mst1_SARAH_domain.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR011524; SARAH_dom.
 IPR002290; Ser/Thr_dual-sp_kinase_dom. 
Pfam
 PF11629; Mst1_SARAH
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS50951; SARAH 
PRINTS