| Tag | Content |
|---|
| CPLM ID | CPLM-019484 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Spermatogenesis-associated protein 13 |
Protein Synonyms/Alias | APC-stimulated guanine nucleotide exchange factor 2; Asef2 |
Gene Name | SPATA13 |
Gene Synonyms/Alias | |
Created Date | July 27, 2013 |
Organism | Homo sapiens (Human) |
NCBI Taxa ID | 9606 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 464 | IHSGELTKITKQGKS | ubiquitination | [1] |
|
Reference | [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA. Mol Cell Proteomics. 2013 Mar;12(3):825-31. [ PMID: 23266961] |
Functional Description | Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Regulates cell migration and adhesion assembly and disassembly through a RAC1, PI3K, RHOA and AKT1-dependent mechanism. Increases both RAC1 and CDC42 activity, but decreases the amount of active RHOA. Required for MMP9 up- regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression. |
Sequence Annotation | DOMAIN 147 206 SH3.
DOMAIN 240 424 DH.
DOMAIN 455 561 PH.
REGION 98 150 ABR (APC-binding region) domain.
REGION 561 652 C-terminal tail. |
Keyword | Alternative splicing; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Membrane; Polymorphism; Reference proteome; SH3 domain. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 652 AA |
Protein Sequence | MTSASPEDQN APVGCPKGAR RRRPISVIGG VSLYGTNQTE ELDNLLTQPA SRPPMPAHQV 60
PPYKAVSARF RPFTFSQSTP IGLDRVGRRR QMRASNVSSD GGTEPSALVD DNGSEEDFSY 120
EDLCQASPRY LQPGGEQLAI NELISDGNVV CAEALWDHVT MDDQELGFKA GDVIQVLEAS 180
NKDWWWGRSE DKEAWFPASF VRLRVNQEEL SENSSSTPSE EQDEEASQSR HRHCENKQQM 240
RTNVIREIMD TERVYIKHLR DICEGYIRQC RKHTGMFTVA QLATIFGNIE DIYKFQRKFL 300
KDLEKQYNKE EPHLSEIGSC FLQNQEGFAI YSEYCNNHPG ACLELANLMK QGKYRHFFEA 360
CRLLQQMIDI AIDGFLLTPV QKICKYPLQL AELLKYTTQE HGDYSNIKAA YEAMKNVACL 420
INERKRKLES IDKIARWQVS IVGWEGLDIL DRSSELIHSG ELTKITKQGK SQQRTFFLFD 480
HQLVSCKKDL LRRDMLYYKG RLDMDEMELV DLGDGRDKDC NLSVKNAFKL VSRTTDEVYL 540
FCAKKQEDKA RWLQACADER RRVQEDKEMG MEISENQKKL AMLNAQKAGH GKSKGYNRCP 600
VAPPHQGLHP IHQRHITMPT SVPQQQVFGL AEPKRKSSLF WHTFNRLTPF RK 652 |
Gene Ontology | GO:0005737; C:cytoplasm; IDA:UniProtKB. GO:0030175; C:filopodium; IDA:UniProtKB. GO:0030027; C:lamellipodium; IDA:UniProtKB. GO:0005634; C:nucleus; IDA:HPA. GO:0032587; C:ruffle membrane; IDA:UniProtKB. GO:0005543; F:phospholipid binding; IEA:InterPro. GO:0030676; F:Rac guanyl-nucleotide exchange factor activity; IDA:UniProtKB. GO:0016477; P:cell migration; IMP:UniProtKB. GO:0046847; P:filopodium assembly; IMP:UniProtKB. GO:0030032; P:lamellipodium assembly; IMP:UniProtKB. GO:0030334; P:regulation of cell migration; IMP:UniProtKB. GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |