CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009052
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 G/T mismatch-specific thymine DNA glycosylase 
Protein Synonyms/Alias
 C-JUN leucine zipper interactive protein JZA-3; Thymine-DNA glycosylase 
Gene Name
 Tdg 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
70EPAPEAPKRRKRKPRacetylation[1]
94PKKPATSKKSGKSTKacetylation[1]
95KKPATSKKSGKSTKSacetylation[1]
98ATSKKSGKSTKSKEKacetylation[1]
Reference
 [1] Association of CBP/p300 acetylase and thymine DNA glycosylase links DNA repair and transcription.
 Tini M, Benecke A, Um SJ, Torchia J, Evans RM, Chambon P.
 Mol Cell. 2002 Feb;9(2):265-77. [PMID: 11864601] 
Functional Description
 DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine (PubMed:21817016). Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC (PubMed:21722948). Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar- phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. 
Sequence Annotation
 CROSSLNK 341 341 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Alternative splicing; Chromatin regulator; Complete proteome; DNA damage; DNA repair; Glycosidase; Hydrolase; Isopeptide bond; Nucleus; Reference proteome; Transcription; Transcription regulation; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 421 AA 
Protein Sequence
MDAEAARSYS LEQVQALYSF PFQQMMAEVP NMAVTTGQQV PAVAPNMATV TEQQVPEDAP 60
VQEPAPEAPK RRKRKPRAAE PQEPVEPKKP ATSKKSGKST KSKEKQEKIT DAFKVKRKVD 120
RFNGVSEAEL LTKTLPDILT FNLDIVIIGI NPGLMAAYKG HHYPGPGNHF WKCLFMSGLS 180
EVQLNHMDDH TLPGKYGIGF TNMVERTTPG SKDLSSKEFR EGGRILVQKL QKYQPRIAVF 240
NGKCIYEIFS KEVFGVKVKN LEFGLQPHKI PDTETLCYVM PSSSARCAQF PRAQDKVHYY 300
IKLKDLRDQL KGIERNADVQ EVQYTFDLQL AQEDAKKMAV KEEKYDPGYE AAYGGAYGEN 360
PCNGEPCGIA SNGLTAHSAE PRGEAAPSDV PNGQWMAQSF AEQIPSFNNC GTREQEEESH 420
A 421 
Gene Ontology
 GO:0005634; C:nucleus; IDA:MGI.
 GO:0019104; F:DNA N-glycosylase activity; IDA:UniProtKB.
 GO:0030983; F:mismatched DNA binding; ISS:UniProtKB.
 GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
 GO:0001104; F:RNA polymerase II transcription cofactor activity; IGI:MGI.
 GO:0006284; P:base-excision repair; IDA:UniProtKB.
 GO:0016568; P:chromatin modification; IEA:UniProtKB-KW.
 GO:0080111; P:DNA demethylation; IDA:UniProtKB.
 GO:0009790; P:embryo development; IMP:UniProtKB.
 GO:0006298; P:mismatch repair; ISS:UniProtKB.
 GO:0035562; P:negative regulation of chromatin binding; IDA:MGI.
 GO:0032091; P:negative regulation of protein binding; IDA:MGI.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:MGI. 
Interpro
 IPR015637; DNA_glycosylase_G/T-mismatch.
 IPR003310; Thymine-DNA_glycosylase.
 IPR005122; Uracil-DNA_glycosylase-like. 
Pfam
 PF03167; UDG 
SMART
 SM00986; UDG 
PROSITE
  
PRINTS