CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010922
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dihydroorotate dehydrogenase (quinone), mitochondrial 
Protein Synonyms/Alias
 DHOdehase; Dihydroorotate oxidase 
Gene Name
 DHODH 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
86EVRVLGHKFRNPVGIubiquitination[1]
169ARQQKQAKLTEDGLPubiquitination[1]
183PLGVNLGKNKTSVDAubiquitination[1]
185GVNLGKNKTSVDAAEubiquitination[1, 2]
226GLRSLQGKAELRRLLubiquitination[1]
235ELRRLLTKVLQERDGubiquitination[1]
306ETGGLSGKPLRDLSTubiquitination[1, 2]
344SGQDALEKIRAGASLubiquitination[1, 2]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor. 
Sequence Annotation
 NP_BIND 95 99 FMN.
 NP_BIND 355 356 FMN.
 REGION 144 148 Substrate binding.
 REGION 211 216 Substrate binding.
 REGION 283 284 Substrate binding.
 ACT_SITE 214 214 Nucleophile.
 BINDING 99 99 Substrate.
 BINDING 119 119 FMN.
 BINDING 180 180 FMN.
 BINDING 211 211 FMN.
 BINDING 254 254 FMN.
 BINDING 282 282 FMN; via carbonyl oxygen.
 BINDING 305 305 FMN; via amide nitrogen.
 BINDING 334 334 FMN; via amide nitrogen.  
Keyword
 3D-structure; Complete proteome; Disease mutation; Flavoprotein; FMN; Membrane; Mitochondrion; Mitochondrion inner membrane; Oxidoreductase; Polymorphism; Pyrimidine biosynthesis; Reference proteome; Transit peptide; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 395 AA 
Protein Sequence
MAWRHLKKRA QDAVIILGGG GLLFASYLMA TGDERFYAEH LMPTLQGLLD PESAHRLAVR 60
FTSLGLLPRA RFQDSDMLEV RVLGHKFRNP VGIAAGFDKH GEAVDGLYKM GFGFVEIGSV 120
TPKPQEGNPR PRVFRLPEDQ AVINRYGFNS HGLSVVEHRL RARQQKQAKL TEDGLPLGVN 180
LGKNKTSVDA AEDYAEGVRV LGPLADYLVV NVSSPNTAGL RSLQGKAELR RLLTKVLQER 240
DGLRRVHRPA VLVKIAPDLT SQDKEDIASV VKELGIDGLI VTNTTVSRPA GLQGALRSET 300
GGLSGKPLRD LSTQTIREMY ALTQGRVPII GVGGVSSGQD ALEKIRAGAS LVQLYTALTF 360
WGPPVVGKVK RELEALLKEQ GFGGVTDAIG ADHRR 395 
Gene Ontology
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005743; C:mitochondrial inner membrane; NAS:UniProtKB.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0004152; F:dihydroorotate dehydrogenase activity; NAS:UniProtKB.
 GO:0004158; F:dihydroorotate oxidase activity; IEA:Compara.
 GO:0008144; F:drug binding; IEA:Compara.
 GO:0010181; F:FMN binding; IEA:Compara.
 GO:0048039; F:ubiquinone binding; IEA:Compara.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; NAS:UniProtKB.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0007565; P:female pregnancy; IEA:Compara.
 GO:0007595; P:lactation; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0046134; P:pyrimidine nucleoside biosynthetic process; TAS:Reactome.
 GO:0090140; P:regulation of mitochondrial fission; IEA:Compara.
 GO:0031000; P:response to caffeine; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR012135; Dihydroorotate_DH_1_2.
 IPR005719; Dihydroorotate_DH_2.
 IPR001295; Dihydroorotate_DH_CS. 
Pfam
 PF01180; DHO_dh 
SMART
  
PROSITE
 PS00911; DHODEHASE_1
 PS00912; DHODEHASE_2 
PRINTS