CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005175
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 3-alpha-hydroxysteroid dehydrogenase 
Protein Synonyms/Alias
 3-alpha-HSD; Hydroxyprostaglandin dehydrogenase 
Gene Name
 Akr1c9 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
31TVPEKVAKDEVIKATacetylation[1]
36VAKDEVIKATKIAIDacetylation[1]
39DEVIKATKIAIDNGFacetylation[1]
101LVRTCLEKTLKSTQLacetylation[1]
201HLYLNQSKMLDYCKSacetylation[1]
225TLGSSRDKTWVDQKSacetylation[1]
246PVLCAIAKKYKQTPAacetylation[1]
275LIRSFNAKRIKELTQacetylation[1]
309NFRYNNAKYFDDHPNacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Besides being a 3-alpha-hydroxysteroid dehydrogenase, the enzyme can accomplish diverse functions: as quinone reductase, as an aromatic alcohol dehydrogenase, as dihydrodiol dehydrogenase, and as 9-, 11-, and 15-hydroxyprostaglandin dehydrogenase. 
Sequence Annotation
 NP_BIND 217 280 NADP.
 ACT_SITE 55 55 Proton donor.
 BINDING 117 117 Substrate.
 MOD_RES 1 1 Blocked amino end (Met).  
Keyword
 3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 322 AA 
Protein Sequence
MDSISLRVAL NDGNFIPVLG FGTTVPEKVA KDEVIKATKI AIDNGFRHFD SAYLYEVEEE 60
VGQAIRSKIE DGTVKREDIF YTSKLWSTFH RPELVRTCLE KTLKSTQLDY VDLYIIHFPM 120
ALQPGDIFFP RDEHGKLLFE TVDICDTWEA MEKCKDAGLA KSIGVSNFNC RQLERILNKP 180
GLKYKPVCNQ VECHLYLNQS KMLDYCKSKD IILVSYCTLG SSRDKTWVDQ KSPVLLDDPV 240
LCAIAKKYKQ TPALVALRYQ LQRGVVPLIR SFNAKRIKEL TQVFEFQLAS EDMKALDGLN 300
RNFRYNNAKY FDDHPNHPFT DE 322 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0047026; F:androsterone dehydrogenase (A-specific) activity; IEA:EC.
 GO:0016229; F:steroid dehydrogenase activity; IMP:RGD.
 GO:0021766; P:hippocampus development; IEP:RGD.
 GO:0008202; P:steroid metabolic process; TAS:RGD. 
Interpro
 IPR001395; Aldo/ket_red.
 IPR018170; Aldo/ket_reductase_CS.
 IPR020471; Aldo/keto_reductase_subgr.
 IPR023210; NADP_OxRdtase_dom. 
Pfam
 PF00248; Aldo_ket_red 
SMART
  
PROSITE
 PS00798; ALDOKETO_REDUCTASE_1
 PS00062; ALDOKETO_REDUCTASE_2
 PS00063; ALDOKETO_REDUCTASE_3 
PRINTS
 PR00069; ALDKETRDTASE.