CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000779
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 LanC-like protein 1 
Protein Synonyms/Alias
 40 kDa erythrocyte membrane protein; p40 
Gene Name
 LANCL1 
Gene Synonyms/Alias
 GPR69A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
15NPYADYNKSLAEGYFubiquitination[1, 2, 3, 4]
101QSLNCLTKRSITFLCubiquitination[3]
128YHKMNNEKQAEDCITubiquitination[1, 2, 3, 4]
142TRLIHLNKIDPHAPNacetylation[5]
142TRLIHLNKIDPHAPNubiquitination[1, 3]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 May play a role in EPS8 signaling. Binds glutathion. 
Sequence Annotation
 REGION 364 367 Glutathione binding.
 METAL 276 276 Zinc.
 METAL 322 322 Zinc.
 METAL 323 323 Zinc.
 BINDING 317 317 Glutathione.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 142 142 N6-acetyllysine.  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; Metal-binding; Nucleus; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 399 AA 
Protein Sequence
MAQRAFPNPY ADYNKSLAEG YFDAAGRLTP EFSQRLTNKI RELLQQMERG LKSADPRDGT 60
GYTGWAGIAV LYLHLYDVFG DPAYLQLAHG YVKQSLNCLT KRSITFLCGD AGPLAVAAVL 120
YHKMNNEKQA EDCITRLIHL NKIDPHAPNE MLYGRIGYIY ALLFVNKNFG VEKIPQSHIQ 180
QICETILTSG ENLARKRNFT AKSPLMYEWY QEYYVGAAHG LAGIYYYLMQ PSLQVSQGKL 240
HSLVKPSVDY VCQLKFPSGN YPPCIGDNRD LLVHWCHGAP GVIYMLIQAY KVFREEKYLC 300
DAYQCADVIW QYGLLKKGYG LCHGSAGNAY AFLTLYNLTQ DMKYLYRACK FAEWCLEYGE 360
HGCRTPDTPF SLFEGMAGTI YFLADLLVPT KARFPAFEL 399 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0004930; F:G-protein coupled receptor activity; TAS:ProtInc.
 GO:0043295; F:glutathione binding; IDA:UniProtKB.
 GO:0050750; F:low-density lipoprotein particle receptor binding; IDA:MGI.
 GO:0008270; F:zinc ion binding; IDA:UniProtKB.
 GO:0008152; P:metabolic process; IEA:GOC. 
Interpro
 IPR008928; 6-hairpin_glycosidase-like.
 IPR012341; 6hp_glycosidase.
 IPR007822; LANC-like.
 IPR020464; LanC-like_prot_euk. 
Pfam
 PF05147; LANC_like 
SMART
  
PROSITE
  
PRINTS
 PR01951; LANCEUKARYTE.
 PR01950; LANCSUPER.