CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-001462
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Retinal dehydrogenase 2 
Protein Synonyms/Alias
 RALDH 2; RalDH2; Aldehyde dehydrogenase family 1 member A2; Retinaldehyde-specific dehydrogenase type 2; RALDH(II) 
Gene Name
 ALDH1A2 
Gene Synonyms/Alias
 RALDH2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
108ERGRLLDKLADLVERubiquitination[1]
269TGSTEVGKLIQEAAGubiquitination[1]
365EQGPQIDKKQYNKILubiquitination[1, 2]
366QGPQIDKKQYNKILEubiquitination[1]
370IDKKQYNKILELIQSubiquitination[1, 2, 3]
384SGVAEGAKLECGGKGubiquitination[1, 2]
390AKLECGGKGLGRKGFubiquitination[1, 2]
395GGKGLGRKGFFIEPTubiquitination[1, 2]
415TDDMRIAKEEIFGPVubiquitination[1, 2]
429VQEILRFKTMDEVIEubiquitination[1, 2]
507LREYSEVKTVTVKIPubiquitination[1]
512EVKTVTVKIPQKNS*ubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Recognizes as substrates free retinal and cellular retinol-binding protein-bound retinal. Does metabolize octanal and decanal but does not metabolize citral, benzaldehyde, acetaldehyde and propanal efficiently (By similarity). 
Sequence Annotation
 NP_BIND 263 268 NAD (By similarity).
 ACT_SITE 286 286 Proton acceptor (By similarity).
 ACT_SITE 320 320 Nucleophile (By similarity).
 MOD_RES 122 122 Phosphothreonine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Cytoplasm; NAD; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 518 AA 
Protein Sequence
MTSSKIEMPG EVKADPAALM ASLHLLPSPT PNLEIKYTKI FINNEWQNSE SGRVFPVYNP 60
ATGEQVCEVQ EADKADIDKA VQAARLAFSL GSVWRRMDAS ERGRLLDKLA DLVERDRAVL 120
ATMESLNGGK PFLQAFYVDL QGVIKTFRYY AGWADKIHGM TIPVDGDYFT FTRHEPIGVC 180
GQIIPWNFPL LMFAWKIAPA LCCGNTVVIK PAEQTPLSAL YMGALIKEAG FPPGVINILP 240
GYGPTAGAAI ASHIGIDKIA FTGSTEVGKL IQEAAGRSNL KRVTLELGGK SPNIIFADAD 300
LDYAVEQAHQ GVFFNQGQCC TAGSRIFVEE SIYEEFVRRS VERAKRRVVG SPFDPTTEQG 360
PQIDKKQYNK ILELIQSGVA EGAKLECGGK GLGRKGFFIE PTVFSNVTDD MRIAKEEIFG 420
PVQEILRFKT MDEVIERANN SDFGLVAAVF TNDINKALTV SSAMQAGTVW INCYNALNAQ 480
SPFGGFKMSG NGREMGEFGL REYSEVKTVT VKIPQKNS 518 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0048471; C:perinuclear region of cytoplasm; IEA:Compara.
 GO:0004028; F:3-chloroallyl aldehyde dehydrogenase activity; ISS:UniProtKB.
 GO:0016918; F:retinal binding; ISS:UniProtKB.
 GO:0001758; F:retinal dehydrogenase activity; ISS:UniProtKB.
 GO:0042904; P:9-cis-retinoic acid biosynthetic process; IEA:Compara.
 GO:0009952; P:anterior/posterior pattern specification; IEA:Compara.
 GO:0001568; P:blood vessel development; IEA:Compara.
 GO:0048738; P:cardiac muscle tissue development; IEA:Compara.
 GO:0071300; P:cellular response to retinoic acid; IEA:Compara.
 GO:0009855; P:determination of bilateral symmetry; IEA:Compara.
 GO:0031076; P:embryonic camera-type eye development; IEA:Compara.
 GO:0048566; P:embryonic digestive tract development; IEA:Compara.
 GO:0035115; P:embryonic forelimb morphogenesis; IEA:Compara.
 GO:0060324; P:face development; IEA:Compara.
 GO:0003007; P:heart morphogenesis; IEA:Compara.
 GO:0030902; P:hindbrain development; IEA:Compara.
 GO:0001822; P:kidney development; IEA:Compara.
 GO:0001889; P:liver development; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0007494; P:midgut development; IEA:Compara.
 GO:0016331; P:morphogenesis of embryonic epithelium; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; IDA:UniProtKB.
 GO:0014032; P:neural crest cell development; IEA:Compara.
 GO:0021915; P:neural tube development; IMP:UniProtKB.
 GO:0030182; P:neuron differentiation; IEA:Compara.
 GO:0031016; P:pancreas development; IEA:Compara.
 GO:0021983; P:pituitary gland development; IEA:Compara.
 GO:0043065; P:positive regulation of apoptotic process; IEA:Compara.
 GO:0008284; P:positive regulation of cell proliferation; IEA:Compara.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0009954; P:proximal/distal pattern formation; IEA:Compara.
 GO:0001936; P:regulation of endothelial cell proliferation; IEA:Compara.
 GO:0034097; P:response to cytokine stimulus; IDA:UniProtKB.
 GO:0032355; P:response to estradiol stimulus; IEA:Compara.
 GO:0033189; P:response to vitamin A; IEA:Compara.
 GO:0042574; P:retinal metabolic process; IEA:Compara.
 GO:0042573; P:retinoic acid metabolic process; ISS:UniProtKB.
 GO:0048384; P:retinoic acid receptor signaling pathway; IEA:Compara.
 GO:0042572; P:retinol metabolic process; IEA:UniProtKB-UniPathway.
 GO:0035799; P:ureter maturation; IEA:Compara.
 GO:0006776; P:vitamin A metabolic process; NAS:UniProtKB. 
Interpro
 IPR016161; Ald_DH/histidinol_DH.
 IPR016163; Ald_DH_C.
 IPR016160; Ald_DH_CS.
 IPR016162; Ald_DH_N.
 IPR015590; Aldehyde_DH_dom. 
Pfam
 PF00171; Aldedh 
SMART
  
PROSITE
 PS00070; ALDEHYDE_DEHYDR_CYS
 PS00687; ALDEHYDE_DEHYDR_GLU 
PRINTS