CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019646
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein IWS1 homolog 
Protein Synonyms/Alias
 IWS1-like protein 
Gene Name
 IWS1 
Gene Synonyms/Alias
 IWS1L 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
600AVVMHLKKQDLKETFacetylation[1]
604HLKKQDLKETFIDSGacetylation[1]
767KDYVVRPKWNVEMESubiquitination[2, 3]
816KISIEGNKMPL****ubiquitination[4]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961
Functional Description
  
Sequence Annotation
 REPEAT 235 260 1.
 REPEAT 261 286 2.
 REPEAT 287 296 3; half-length.
 DOMAIN 614 692 TFIIS N-terminal.
 REGION 235 296 3 X approximate tandem repeats.
 MOD_RES 69 69 Phosphoserine.
 MOD_RES 93 93 Phosphoserine.
 MOD_RES 95 95 Phosphoserine.
 MOD_RES 157 157 Phosphoserine.
 MOD_RES 159 159 Phosphoserine.
 MOD_RES 196 196 Phosphoserine.
 MOD_RES 198 198 Phosphoserine.
 MOD_RES 209 209 Phosphoserine (By similarity).
 MOD_RES 235 235 Phosphoserine.
 MOD_RES 237 237 Phosphoserine.
 MOD_RES 261 261 Phosphoserine.
 MOD_RES 263 263 Phosphoserine.
 MOD_RES 287 287 Phosphoserine.
 MOD_RES 289 289 Phosphoserine.
 MOD_RES 300 300 Phosphoserine.
 MOD_RES 302 302 Phosphoserine.
 MOD_RES 304 304 Phosphoserine.
 MOD_RES 313 313 Phosphoserine.
 MOD_RES 315 315 Phosphoserine.
 MOD_RES 329 329 Phosphoserine.
 MOD_RES 333 333 Phosphoserine.
 MOD_RES 363 363 Phosphoserine.
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 377 377 Phosphoserine.
 MOD_RES 398 398 Phosphoserine.
 MOD_RES 400 400 Phosphoserine.
 MOD_RES 415 415 Phosphoserine.
 MOD_RES 420 420 Phosphoserine.
 MOD_RES 422 422 Phosphoserine.
 MOD_RES 426 426 Phosphoserine (By similarity).
 MOD_RES 435 435 Phosphothreonine.
 MOD_RES 438 438 Phosphoserine.
 MOD_RES 440 440 Phosphoserine.
 MOD_RES 511 511 Phosphoserine (By similarity).
 MOD_RES 513 513 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 819 AA 
Protein Sequence
MDSEYYSGDQ SDDGGATPVQ DERDSGSDGE DDVNEQHSGS DTGSVERHSE NETSDREDGL 60
PKGHHVTDSE NDEPLNLNAS DSESEELHRQ KDSDSESEER AEPPASDSEN EDVNQHGSDS 120
ESEETRKLPG SDSENEELLN GHASDSENED VGKHPASDSE IEELQKSPAS DSETEDALKP 180
QISDSESEEP PRHQASDSEN EEPPKPRMSD SESEELPKPQ VSDSESEEPP RHQASDSENE 240
ELPKPRISDS ESEDPPRHQA SDSENEELPK PRISDSESED PPRNQASDSE NEELPKPRVS 300
DSESEGPQKG PASDSETEDA SRHKQKPESD DDSDRENKGE DTEMQNDSFH SDSHMDRKKF 360
HSSDSEEEEH KKQKMDSDED EKEGEEEKVA KRKAAVLSDS EDEEKASAKK SRVVSDADDS 420
DSDAVSDKSG KREKTIASDS EEEAGKELSD KKNEEKDLFG SDSESGNEEE NLIADIFGES 480
GDEEEEEFTG FNQEDLEEEK GETQVKEAED SDSDDNIKRG KHMDFLSDFE MMLQRKKSMS 540
GKRRRNRDGG TFISDADDVV SAMIVKMNEA AEEDRQLNNQ KKPALKKLTL LPAVVMHLKK 600
QDLKETFIDS GVMSAIKEWL SPLPDRSLPA LKIREELLKI LQELPSVSQE TLKHSGIGRA 660
VMYLYKHPKE SRSNKDMAGK LINEWSRPIF GLTSNYKGMT REEREQRDLE QMPQRRRMNS 720
TGGQTPRRDL EKVLTGEEKA LRPGDPGFCA RARVPMPSNK DYVVRPKWNV EMESSRFQAT 780
SKKGISRLDK QMRKFTDIRK KSRSAHAVKI SIEGNKMPL 819 
Gene Ontology
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0006351; P:transcription, DNA-dependent; IEA:InterPro. 
Interpro
 IPR017923; TFIIS_N. 
Pfam
 PF08711; Med26 
SMART
  
PROSITE
 PS51319; TFIIS_N 
PRINTS