UniProt Accession

 RNF4_HUMAN; P78317; B2R6D6; D6RF58; Q49AR8 

Genbank Protein ID

 AB000468; U95140; AK128038; AK309509; AK312534; AL110117; AL645924; BX322586; CR450722; CR545473; BC031935 

Genbank Nucleotide ID

 BAA19122.1; AAC52022.1; BAG54620.1; BAG35433.1; AAH31935.1 

Protein Name

 E3 ubiquitin-protein ligase RNF4 

Protein Synonyms/Alias

 RING finger protein 4; Small nuclear ring finger protein; Protein SNURF 

Gene Name

 RNF4 

Gene Synonyms/Alias

 SNURF; RES4-26 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
168	QCLRDSLKNANTCPT	ubiquitination	[1]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor- mediated transcriptional activation. 

Sequence Annotation

 ZN_FING 132 177 RING-type.
 REGION 1 16 Required for ubiquitination activity (By
 REGION 4 61 Mediates interaction with TRPS1 (By
 MOTIF 36 39 SUMO interaction motif 1; mediates the
 MOTIF 46 49 SUMO interaction motif 2; mediates the
 MOTIF 57 59 SUMO interaction motif 3; mediates the
 MOTIF 67 70 SUMO interaction motif 4; mediates the
 MOD_RES 94 94 Phosphoserine.
 MOD_RES 95 95 Phosphoserine.  

Keyword

 3D-structure; Activator; Alternative splicing; Complete proteome; Cytoplasm; DNA-binding; Ligase; Metal-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Ubl conjugation; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 190 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005634; C:nucleus; IDA:UniProtKB.
 GO:0016605; C:PML body; IEA:UniProtKB-SubCell.
 GO:0050681; F:androgen receptor binding; NAS:UniProtKB.
 GO:0003677; F:DNA binding; ISS:UniProtKB.
 GO:0031491; F:nucleosome binding; ISS:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:ProtInc.
 GO:0032184; F:SUMO polymer binding; ISS:UniProtKB.
 GO:0003713; F:transcription coactivator activity; NAS:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; TAS:ProtInc.
 GO:0030521; P:androgen receptor signaling pathway; NAS:UniProtKB.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0051865; P:protein autoubiquitination; ISS:UniProtKB.
 GO:0070979; P:protein K11-linked ubiquitination; ISS:UniProtKB.
 GO:0070936; P:protein K48-linked ubiquitination; ISS:UniProtKB.
 GO:0085020; P:protein K6-linked ubiquitination; ISS:UniProtKB.
 GO:0070534; P:protein K63-linked ubiquitination; ISS:UniProtKB.
 GO:0090234; P:regulation of kinetochore assembly; IMP:UniProtKB.
 GO:0090169; P:regulation of spindle assembly; IMP:UniProtKB.
 GO:0046685; P:response to arsenic-containing substance; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD.
 IPR017907; Znf_RING_CS. 

Pfam

 PF13639; zf-RING_2 

SMART

 SM00184; RING 

PROSITE

 PS00518; ZF_RING_1
 PS50089; ZF_RING_2 

PRINTS