CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000368
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
 BAD08351.1; BAD08352.1; BAA20817.1; BAG58097.1; BAG58528.1; CAI94923.1; CAI94923.1; CAI94923.1; CAI94923.1; CAI94928.1; CAI94928.1; CAI94928.1; CAI94928.1; CAI95103.1; CAI95103.1; CAI95103.1; CAI95103.1; CAI95400.1; CAI95400.1; CAI95400.1; CAI95400.1; CAH70243.1; CAH70243.1; CAH70243.1; CAH70243.1; CAH70244.2; CAH70244.2; CAH70244.2; CAH70244.2; CAH70245.1; CAH70245.1; CAH70245.1; CAH70245.1; CAI39523.1; CAI39523.1; CAI39523.1; CAI39523.1; CAI39524.2; CAI39524.2; CAI39524.2; CAI39524.2; CAI39525.1; CAI39525.1; CAI39525.1; CAI39525.1; CAI39869.1; CAI39869.1; CAI39869.1; CAI39869.1; CAI39870.2; CAI39870.2; CAI39870.2; CAI39870.2; CAI39871.1; CAI39871.1; CAI39871.1; CAI39871.1; CAM13074.1; CAM13074.1; CAM13075.1; CAI41383.1; CAI41383.1; CAI41383.1; CAI41383.1; CAM13089.1; CAM13089.1; CAI41390.2; CAI41390.2; CAI41390.2; CAI41390.2; CAI41392.1; CAI41392.1; CAI41392.1; CAI41392.1; CAM24069.3; CAM24070.1; AAH11853.1; AAH20208.1 
Protein Name
 Guanine nucleotide exchange factor DBS 
Protein Synonyms/Alias
 DBL's big sister; MCF2-transforming sequence-like protein 
Gene Name
 MCF2L 
Gene Synonyms/Alias
 KIAA0362 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
209DLHGYIDKSQLTEDLubiquitination[1]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Guanine nucleotide exchange factor that potentially links pathways that signal through RAC1, RHOA and CDC42. Catalyzes guanine nucleotide exchange on RHOA and CDC42 and interacts specifically with the GTP-bound form of RAC1, suggesting that it functions as an effector of RAC1. May also participate in axonal transport in the brain. Becomes activated and highly tumorigenic by truncation of the N-terminus (By similarity). Isoform 5 activates CDC42. 
Sequence Annotation
 DOMAIN 52 224 CRAL-TRIO.
 REPEAT 351 456 Spectrin.
 DOMAIN 631 811 DH.
 DOMAIN 829 945 PH.
 DOMAIN 1055 1116 SH3.
 MOD_RES 457 457 Phosphoserine (By similarity).
 MOD_RES 480 480 Phosphoserine (By similarity).  
Keyword
 Alternative splicing; Cell membrane; Coiled coil; Complete proteome; Cytoplasm; Guanine-nucleotide releasing factor; Membrane; Phosphoprotein; Proto-oncogene; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1137 AA 
Protein Sequence
MFDCWRFILC KRPGSNSYSS PQRPNEAKKE ETDHQIDVSD VIRLVQDTPE ATAMATDEIM 60
HQDIVPLCAA DIQDQLKKRF AYLSGGRGQD GSPVITFPDY PAFSEIPDKE FQNVMTYLTS 120
IPSLQDAGIG FILVIDRRRD KWTSVKASVL RIAASFPANL QLVLVLRPTG FFQRTLSDIA 180
FKFNRDDFKM KVPVIMLSSV PDLHGYIDKS QLTEDLGGTL DYCHSRWLCQ RTAIESFALM 240
VKQTAQMLQS FGTELAETEL PNDVQSTSSV LCAHTEKKDK AKEDLRLALK EGHSVLESLR 300
ELQAEGSEPS VNQDQLDNQA TVQRLLAQLN ETEAAFDEFW AKHQQKLEQC LQLRHFEQGF 360
REVKAILDAA SQKIATFTDI GNSLAHVEHL LRDLASFEEK SGVAVERARA LSLDGEQLIG 420
NKHYAVDSIR PKCQELRHLC DQFSAEIARR RGLLSKSLEL HRRLETSMKW CDEGIYLLAS 480
QPVDKCQSQD GAEAALQEIE KFLETGAENK IQELNAIYKE YESILNQDLM EHVRKVFQKQ 540
ASMEEVFHRR QASLKKLAAR QTRPVQPVAP RPEALAKSPC PSPGIRRGSE NSSSEGGALR 600
RGPYRRAKSE MSESRQGRGS AGEEEESLAI LRRHVMSELL DTERAYVEEL LCVLEGYAAE 660
MDNPLMAHLL STGLHNKKDV LFGNMEEIYH FHNRIFLREL ENYTDCPELV GRCFLERMED 720
FQIYEKYCQN KPRSESLWRQ CSDCPFFQEC QRKLDHKLSL DSYLLKPVQR ITKYQLLLKE 780
MLKYSRNCEG AEDLQEALSS ILGILKAVND SMHLIAITGY DGNLGDLGKL LMQGSFSVWT 840
DHKRGHTKVK ELARFKPMQR HLFLHEKAVL FCKKREENGE GYEKAPSYSY KQSLNMAAVG 900
ITENVKGDAK KFEIWYNARE EVYIVQAPTP EIKAAWVNEI RKVLTSQLQA CREASQHRAL 960
EQSQSLPLPA PTSTSPSRGN SRNIKKLEER KTDPLSLEGY VSSAPLTKPP EKGKGWSKTS 1020
HSLEAPEDDG GWSSAEEQIN SSDAEEDGGL GPKKLVPGKY TVVADHEKGG PDALRVRSGD 1080
VVELVQEGDE GLWYVRDPTT GKEGWVPASS LSVRLGPSGS AQCLSSSGKA HVPRAHP 1137 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; IEA:Compara.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005545; F:1-phosphatidylinositol binding; IEA:Compara.
 GO:0005089; F:Rho guanyl-nucleotide exchange factor activity; IEA:Compara.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0035023; P:regulation of Rho protein signal transduction; IEA:InterPro.
 GO:0051056; P:regulation of small GTPase mediated signal transduction; TAS:Reactome.
 GO:0007266; P:Rho protein signal transduction; IEA:Compara.
 GO:0007264; P:small GTPase mediated signal transduction; TAS:Reactome. 
Interpro
 IPR001251; CRAL-TRIO_dom.
 IPR000219; DH-domain.
 IPR001331; GDS_CDC24_CS.
 IPR011993; PH_like_dom.
 IPR001849; Pleckstrin_homology.
 IPR011511; SH3_2.
 IPR001452; SH3_domain.
 IPR018159; Spectrin/alpha-actinin.
 IPR002017; Spectrin_repeat. 
Pfam
 PF00169; PH
 PF00621; RhoGEF
 PF07653; SH3_2
 PF00435; Spectrin 
SMART
 SM00233; PH
 SM00325; RhoGEF
 SM00516; SEC14
 SM00326; SH3
 SM00150; SPEC 
PROSITE
 PS50191; CRAL_TRIO
 PS00741; DH_1
 PS50010; DH_2
 PS50003; PH_DOMAIN
 PS50002; SH3 
PRINTS