UniProt Accession

 ARH_HUMAN; Q5SW96; A2BHI5; Q6TQS9; Q8N2Y0; Q9UFI9 

Genbank Protein ID

 AY389348; AL117654; AL606491; BX572623; BX572623; AL606491; BC029770 

Genbank Nucleotide ID

 AAQ90407.1; CAB56030.2; CAI16483.1; CAI16483.1; CAM12863.1; CAM12863.1; AAH29770.2 

Protein Name

 Low density lipoprotein receptor adapter protein 1 

Protein Synonyms/Alias

 Autosomal recessive hypercholesterolemia protein 

Gene Name

 LDLRAP1 

Gene Synonyms/Alias

 ARH 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
5	***MDALKSAGRALI	ubiquitination	[1]
184	EEKEKRDKASQEGGD	ubiquitination	[2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Adapter protein (clathrin-associated sorting protein (CLASP)) required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). May be required for LDL binding and internalization but not for receptor clustering in coated pits. May facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits. May also be involved in the internalization of other LDLR family members. Binds to phosphoinositides, which regulate clathrin bud assembly at the cell surface. 

Sequence Annotation

 DOMAIN 42 196 PID.
 REGION 249 276 AP-2 complex binding.
 MOTIF 212 216 Clathrin box.
 MOTIF 257 266 [DE]-X(1,2)-F-X-X-[FL]-X-X-X-R motif.
 MOD_RES 14 14 Phosphoserine.  

Keyword

 3D-structure; Atherosclerosis; Cholesterol metabolism; Complete proteome; Cytoplasm; Disease mutation; Endocytosis; Hyperlipidemia; Lipid metabolism; Phosphoprotein; Polymorphism; Reference proteome; Steroid metabolism; Sterol metabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 308 AA 

Protein Sequence

Gene Ontology

 GO:0009925; C:basal plasma membrane; IDA:UniProtKB.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005769; C:early endosome; IDA:UniProtKB.
 GO:0009898; C:internal side of plasma membrane; IDA:BHF-UCL.
 GO:0005883; C:neurofilament; ISS:BHF-UCL.
 GO:0055037; C:recycling endosome; IDA:BHF-UCL.
 GO:0035612; F:AP-2 adaptor complex binding; IDA:BHF-UCL.
 GO:0001540; F:beta-amyloid binding; IPI:BHF-UCL.
 GO:0035615; F:clathrin adaptor activity; IDA:BHF-UCL.
 GO:0005546; F:phosphatidylinositol-4,5-bisphosphate binding; IDA:BHF-UCL.
 GO:0001784; F:phosphotyrosine binding; IDA:UniProtKB.
 GO:0030159; F:receptor signaling complex scaffold activity; IMP:UniProtKB.
 GO:0035591; F:signaling adaptor activity; IDA:BHF-UCL.
 GO:0042982; P:amyloid precursor protein metabolic process; IMP:BHF-UCL.
 GO:0042632; P:cholesterol homeostasis; IMP:BHF-UCL.
 GO:0008203; P:cholesterol metabolic process; NAS:UniProtKB.
 GO:0090205; P:positive regulation of cholesterol metabolic process; IC:BHF-UCL.
 GO:0048260; P:positive regulation of receptor-mediated endocytosis; IMP:UniProtKB.
 GO:0031623; P:receptor internalization; IMP:BHF-UCL.
 GO:0090118; P:receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport; IMP:BHF-UCL.
 GO:0090003; P:regulation of establishment of protein localization to plasma membrane; IMP:BHF-UCL.
 GO:0043393; P:regulation of protein binding; IMP:UniProtKB. 

Interpro

 IPR011993; PH_like_dom.
 IPR006020; PTyr_interaction_dom. 

Pfam

 PF00640; PID 

SMART

 SM00462; PTB 

PROSITE

 PS01179; PID 

PRINTS