CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043528
UniProt Accession
Genbank Protein ID
 Z92544 
Genbank Nucleotide ID
  
Protein Name
 Mitochondrial Rho GTPase 2 
Protein Synonyms/Alias
  
Gene Name
 RHOT2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
75EIHKIRTKWIPLVNGubiquitination[1]
98PIILVGNKSDLRSGSubiquitination[1, 2, 3, 4, 5]
143ELFYYAQKAVLHPTAubiquitination[1, 5]
158PLYDPEAKQLRPACAubiquitination[1, 2, 5]
193EELNAFQKSCFGHPLubiquitination[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; GTP-binding; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 208 AA 
Protein Sequence
DVRILLLGEA QVGKTSLILS LVGEEFPEEV PPRAEEITIP ADVTPEKVPT HIVDYSEAEQ 60
TDEELREEIH KIRTKWIPLV NGGTTQGPRV PIILVGNKSD LRSGSSMEAV LPIMSQFPEI 120
ETCVECSAKN LRNISELFYY AQKAVLHPTA PLYDPEAKQL RPACAQALTR IFRLSDQDLD 180
QALSDEELNA FQKSCFGHPL APQALEDV 208 
Gene Ontology
 GO:0005622; C:intracellular; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR020860; MIRO.
 IPR013684; MIRO-like.
 IPR027417; P-loop_NTPase.
 IPR001806; Small_GTPase. 
Pfam
 PF08477; Miro
 PF00071; Ras 
SMART
  
PROSITE
 PS51423; MIRO 
PRINTS
 PR00449; RASTRNSFRMNG.