CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012265
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin-associated protein 2-like 
Protein Synonyms/Alias
 Protein NICE-4 
Gene Name
 UBAP2L 
Gene Synonyms/Alias
 KIAA0144; NICE4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
26NQNQTQHKQRPQATAubiquitination[1]
290DLAVLLGKTPSTMENubiquitination[2]
344SMVSMLGKGFGDVGEubiquitination[1]
353FGDVGEAKGGSTTGSubiquitination[1, 2]
420AVHSPFTKRQAFTPSubiquitination[1]
864PYSGDLTKFGRGDASubiquitination[1, 2, 3]
970HGYNTGRKYPPPYKHubiquitination[1, 4]
976RKYPPPYKHFWTAESacetylation[5, 6]
976RKYPPPYKHFWTAESubiquitination[3, 7]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [6] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473
Functional Description
  
Sequence Annotation
 DOMAIN 49 89 UBA.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 416 416 Phosphoserine.
 MOD_RES 425 425 Phosphothreonine.
 MOD_RES 439 439 Phosphoserine.
 MOD_RES 454 454 Phosphoserine.
 MOD_RES 467 467 Phosphoserine.
 MOD_RES 470 470 Phosphoserine.
 MOD_RES 471 471 Phosphoserine.
 MOD_RES 477 477 Phosphoserine.
 MOD_RES 493 493 Phosphoserine.
 MOD_RES 605 605 Phosphoserine.
 MOD_RES 608 608 Phosphoserine.
 MOD_RES 609 609 Phosphoserine.
 MOD_RES 852 852 Phosphoserine.
 MOD_RES 859 859 Phosphoserine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1087 AA 
Protein Sequence
MMTSVGTNRA RGNWEQPQNQ NQTQHKQRPQ ATAEQIRLAQ MISDHNDADF EEKVKQLIDI 60
TGKNQDECVI ALHDCNGDVN RAINVLLEGN PDTHSWEMVG KKKGVSGQKD GGQTESNEEG 120
KENRDRDRDY SRRRGGPPRR GRGASRGREF RGQENGLDGT KSGGPSGRGT ERGRRGRGRG 180
RGGSGRRGGR FSAQGMGTFN PADYAEPANT DDNYGNSSGN TWNNTGHFEP DDGTSAWRTA 240
TEEWGTEDWN EDLSETKIFT ASNVSSVPLP AENVTITAGQ RIDLAVLLGK TPSTMENDSS 300
NLDPSQAPSL AQPLVFSNSK QTAISQPASG NTFSHHSMVS MLGKGFGDVG EAKGGSTTGS 360
QFLEQFKTAQ ALAQLAAQHS QSGSTTTSSW DMGSTTQSPS LVQYDLKNPS DSAVHSPFTK 420
RQAFTPSSTM MEVFLQEKSP AVATSTAAPP PPSSPLPSKS TSAPQMSPGS SDNQSSSPQP 480
AQQKLKQQKK KASLTSKIPA LAVEMPGSAD ISGLNLQFGA LQFGSEPVLS DYESTPTTSA 540
SSSQAPSSLY TSTASESSST ISSNQSQESG YQSGPIQSTT YTSQNNAQGP LYEQRSTQTR 600
RYPSSISSSP QKDLTQAKNG FSSVQATQLQ TTQSVEGATG SAVKSDSPST SSIPPLNETV 660
SAASLLTTTN QHSSSLGGLS HSEEIPNTTT TQHSSTLSTQ QNTLSSSTSS GRTSTSTLLH 720
TSVESEANLH SSSSTFSTTS STVSAPPPVV SVSSSLNSGS SLGLSLGSNS TVTASTRSSV 780
ATTSGKAPPN LPPGVPPLLP NPYIMAPGLL HAYPPQVYGY DDLQMLQTRF PLDYYSIPFP 840
TPTTPLTGRD GSLASNPYSG DLTKFGRGDA SSPAPATTLA QPQQNQTQTH HTTQQTFLNP 900
ALPPGYSYTS LPYYTGVPGL PSTFQYGPAV FPVAPTSSKQ HGVNVSVNAS ATPFQQPSGY 960
GSHGYNTGRK YPPPYKHFWT AES 983 
Gene Ontology
 GO:0007339; P:binding of sperm to zona pellucida; IMP:UniProtKB. 
Interpro
 IPR022166; DUF3697_Uba2.
 IPR009060; UBA-like.
 IPR015940; UBA/transl_elong_EF1B_N_euk. 
Pfam
 PF12478; DUF3697 
SMART
 SM00165; UBA 
PROSITE
 PS50030; UBA 
PRINTS