UniProt Accession

 SF01_HUMAN; Q15637; B7Z1Q1; C9JJE2; Q14818; Q14819; Q15913; Q8IY00; Q92744; Q92745; Q969H7; Q9BW01; Q9UEI0 

Genbank Protein ID

 Y08765; Y08766; L49345; L49380; D26120; D26120; AK293753; AP001462; BC000773; BC008080; BC008724; BC011657; BC020217; BC038446; AJ000051; AJ000052 

Genbank Nucleotide ID

 CAA70018.1; CAA70019.1; AAB03514.1; AAB04033.1; BAA05116.1; BAA05117.1; BAH11587.1; AAH00773.1; AAH08080.1; AAH08724.1; AAH20217.1; AAH38446.1; CAA03883.1; CAA03883.1 

Protein Name

 Splicing factor 1 

Protein Synonyms/Alias

 Mammalian branch point-binding protein; BBP; mBBP; Transcription factor ZFM1; Zinc finger gene in MEN1 locus; Zinc finger protein 162 

Gene Name

 SF1 

Gene Synonyms/Alias

 ZFM1; ZNF162 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
15	TPLDFPSKKRKRSRW	ubiquitination	[1]
16	PLDFPSKKRKRSRWN	ubiquitination	[1]
217	NTMENVKKAVEQIRN	acetylation	[2]
227	EQIRNILKQGIETPE	ubiquitination	[1, 3, 4]
306	DPQSAQDKARMDKEY	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor. 

Sequence Annotation

 DOMAIN 141 222 KH.
 ZN_FING 277 296 CCHC-type.
 MOTIF 15 19 Nuclear localization signal (Potential).
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 20 20 Phosphoserine; by PKG.
 MOD_RES 80 80 Phosphoserine.
 MOD_RES 82 82 Phosphoserine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; Metal-binding; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; RNA-binding; Spliceosome; Transcription; Transcription regulation; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 639 AA 

Protein Sequence

Gene Ontology

 GO:0005840; C:ribosome; NAS:UniProtKB.
 GO:0005681; C:spliceosomal complex; IDA:HGNC.
 GO:0003723; F:RNA binding; TAS:UniProtKB.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0022402; P:cell cycle process; IEA:Compara.
 GO:0033327; P:Leydig cell differentiation; IEA:Compara.
 GO:0030238; P:male sex determination; IEA:Compara.
 GO:0000389; P:mRNA 3'-splice site recognition; TAS:HGNC.
 GO:0050810; P:regulation of steroid biosynthetic process; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004087; KH_dom.
 IPR004088; KH_dom_type_1.
 IPR001878; Znf_CCHC. 

Pfam

 PF00013; KH_1
 PF00098; zf-CCHC 

SMART

 SM00322; KH
 SM00343; ZnF_C2HC 

PROSITE

 PS50084; KH_TYPE_1
 PS50158; ZF_CCHC 

PRINTS