CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-031288
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Protein arginine N-methyltransferase 5 
Protein Synonyms/Alias
 cDNA FLJ54039, highly similar to Protein arginine N-methyltransferase 5 (EC 2.1.1.-) 
Gene Name
 PRMT5 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
94RTLCDYSKRIAVALEubiquitination[1]
121RWLGEPIKAAILPTSubiquitination[1, 2, 3, 4, 5, 6]
134TSIFLTNKKGFPVLSubiquitination[1, 2, 3, 5]
135SIFLTNKKGFPVLSKubiquitination[1, 6]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Methyltransferase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 531 AA 
Protein Sequence
MLQELNFGAY LGLPAFLLPL NQEDNTNLAR VLTNHIHTGH HSSMFWMRVP LVAPEDLRDD 60
IIENAPTTHT EEYSGEEKTW MWWHNFRTLC DYSKRIAVAL EIGADLPSNH VIDRWLGEPI 120
KAAILPTSIF LTNKKGFPVL SKMHQRLIFR LLKLEVQFII TGTNHHSEKE FCSYLQYLEY 180
LSQNRPPPNA YELFAKGYED YLQSPLQPLM DNLESQTYEV FEKDPIKYSQ YQQAIYKCLL 240
DRVPEEEKDT NVQVLMVLGA GRGPLVNASL RAAKQADRRI KLYAVEKNPN AVVTLENWQF 300
EEWGSQVTVV SSDMREWVAP EKADIIVSEL LGSFADNELS PECLDGAQHF LKDDGVSIPG 360
EYTSFLAPIS SSKLYNEVRA CREKDRDPEA QFEMPYVVRL HNFHQLSAPQ PCFTFSHPNR 420
DPMIDNNRYC TLEFPVEVNT VLHGFAGYFE TVLYQDITLS IRPETHSPGM FSWFPILFPI 480
KQPITVREGQ TICVRFWRCS NSKKVWYEWA VTAPVCSAIH NPTGRSYTIG L 531 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IEA:Compara.
 GO:0006479; P:protein methylation; IEA:InterPro. 
Interpro
 IPR025799; Arg_MeTrfase.
 IPR007857; Arg_MeTrfase_PRMT5. 
Pfam
 PF05185; PRMT5 
SMART
  
PROSITE
 PS51632; SAM_PRMT_3 
PRINTS