CPLM-010431

Genbank Nucleotide ID

Biogenesis of lysosome-related organelles complex 1 subunit 1

Protein Synonyms/Alias

BLOC-1 subunit 1; GCN5-like protein 1; Protein RT14

Homo sapiens (Human)

Position	Peptide	Type	References
145	TALEYVYKGQLQSAP	ubiquitination	[1, 2, 3, 4, 5]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]

Functional Description

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. May negatively regulate aerobic respiration through mitochondrial protein lysine-acetylation. May counteract the action of the deacetylase SIRT3 by acetylating and regulating proteins of the mitochondrial respiratory chain including ATP5A1 and NDUFA9.

Alternative initiation; Coiled coil; Complete proteome; Cytoplasm; Mitochondrion; Reference proteome.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0031083; C:BLOC-1 complex; IDA:UniProtKB.
GO:0005765; C:lysosomal membrane; TAS:Reactome.
GO:0005758; C:mitochondrial intermembrane space; IDA:UniProtKB.
GO:0005759; C:mitochondrial matrix; IDA:UniProtKB.
GO:0009060; P:aerobic respiration; IMP:UniProtKB.
GO:0008089; P:anterograde axon cargo transport; ISS:UniProtKB.
GO:0048490; P:anterograde synaptic vesicle transport; ISS:UniProtKB.
GO:0016044; P:cellular membrane organization; TAS:Reactome.
GO:0032438; P:melanosome organization; NAS:UniProtKB.
GO:0031175; P:neuron projection development; ISS:UniProtKB.
GO:0018394; P:peptidyl-lysine acetylation; IMP:UniProtKB.
GO:0060155; P:platelet dense granule organization; NAS:UniProtKB.
GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.