CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000340
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Antizyme inhibitor 1 
Protein Synonyms/Alias
 AZI; Ornithine decarboxylase antizyme inhibitor 
Gene Name
 AZIN1 
Gene Synonyms/Alias
 OAZI; OAZIN 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
47FFVGDLGKIVKKHSQubiquitination[1, 2, 3]
50GDLGKIVKKHSQWQNubiquitination[2]
51DLGKIVKKHSQWQNVubiquitination[2, 3]
63QNVVAQIKPFYTVKCubiquitination[2, 3]
121CKQVSQIKYAAKVGVubiquitination[2]
125SQIKYAAKVGVNILTubiquitination[2]
140CDNEIELKKIARNHPubiquitination[2, 4]
141DNEIELKKIARNHPNubiquitination[2]
150ARNHPNAKVLLHIATubiquitination[2, 3]
169GGEEGNMKFGTTLKNubiquitination[2, 3]
175MKFGTTLKNCRHLLEubiquitination[1, 2, 3, 4, 5, 6]
203FHVSSACKESQVYVHubiquitination[2, 4]
291AVNIIAKKVVENDKFubiquitination[2]
297KKVVENDKFPSGVEKubiquitination[1, 2]
327VYGSFASKLSEDLNTubiquitination[2, 3]
340NTIPEVHKKYKEDEPubiquitination[1, 2, 3]
341TIPEVHKKYKEDEPLubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Regulates cellular polyamine homeostasis (By similarity). Inhibits antizyme-dependent ornithine decarboxylase degradation by binding to antizyme. 
Sequence Annotation
  
Keyword
 Complete proteome; Polyamine biosynthesis; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 448 AA 
Protein Sequence
MKGFIDDANY SVGLLDEGTN LGNVIDNYVY EHTLTGKNAF FVGDLGKIVK KHSQWQNVVA 60
QIKPFYTVKC NSAPAVLEIL AALGTGFACS SKNEMALVQE LGVPPENIIY ISPCKQVSQI 120
KYAAKVGVNI LTCDNEIELK KIARNHPNAK VLLHIATEDN IGGEEGNMKF GTTLKNCRHL 180
LECAKELDVQ IIGVKFHVSS ACKESQVYVH ALSDARCVFD MAGEIGFTMN MLDIGGGFTG 240
TEFQLEEVNH VISPLLDIYF PEGSGVKIIS EPGSYYVSSA FTLAVNIIAK KVVENDKFPS 300
GVEKTGSDEP AFMYYMNDGV YGSFASKLSE DLNTIPEVHK KYKEDEPLFT SSLWGPSCDE 360
LDQIVESCLL PELNVGDWLI FDNMGADSFH EPSAFNDFQR PAIYYMMSFS DWYEMQDAGI 420
TSDSMMKNFF FVPSCIQLSQ EDSFSAEA 448 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0003824; F:catalytic activity; IEA:InterPro.
 GO:0004857; F:enzyme inhibitor activity; TAS:ProtInc.
 GO:0042978; F:ornithine decarboxylase activator activity; IEA:Compara.
 GO:0034641; P:cellular nitrogen compound metabolic process; TAS:Reactome.
 GO:0006596; P:polyamine biosynthetic process; IEA:UniProtKB-KW.
 GO:0006521; P:regulation of cellular amino acid metabolic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 
Interpro
 IPR009006; Ala_racemase/Decarboxylase_C.
 IPR022643; De-COase2_C.
 IPR022657; De-COase2_CS.
 IPR022644; De-COase2_N.
 IPR000183; Orn/DAP/Arg_de-COase.
 IPR002433; Orn_de-COase. 
Pfam
 PF02784; Orn_Arg_deC_N
 PF00278; Orn_DAP_Arg_deC 
SMART
  
PROSITE
 PS00878; ODR_DC_2_1
 PS00879; ODR_DC_2_2 
PRINTS
 PR01179; ODADCRBXLASE.
 PR01182; ORNDCRBXLASE.