CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043663
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial Rho GTPase 
Protein Synonyms/Alias
  
Gene Name
 RHOT1 
Gene Synonyms/Alias
 hCG_1991479 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
132ECSAKNLKNISELFYubiquitination[1, 2]
161CPEEKEMKPACIKALubiquitination[3]
166EMKPACIKALTRIFKubiquitination[1, 3, 4, 5]
173KALTRIFKISDQDNDubiquitination[2]
214DVKNVVRKHISDGVAubiquitination[2]
491EYCARIFKQHFMDSRubiquitination[5]
551PSKDIFVKLTTMAMYubiquitination[2]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Mitochondrial GTPase involved in mitochondrial trafficking (By similarity). 
Sequence Annotation
  
Keyword
 Calcium; Complete proteome; GTP-binding; Hydrolase; Membrane; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MSLVSEEFPE EVPPRAEEIT IPADVTPERV PTHIVDYSEA EQSDEQLHQE ISQANVICIV 60
YAVNNKHSID KVTSRWIPLI NERTDKDSRL PLILVGNKSD LVEYSSMETI LPIMNQYTEI 120
ETCVECSAKN LKNISELFYY AQKAVLHPTG PLYCPEEKEM KPACIKALTR IFKISDQDND 180
GTLNDAELNF FQRICFNTPL APQALEDVKN VVRKHISDGV ADSGLTLKGF LFLHTLFIQR 240
GRHETTWTVL RRFGYDDDLD LTPEYLFPLL KIPPDCTTEL NHHAYLFLQS TFDKHDLDRD 300
CALSPDELKD LFKVFPYIPW GPDVNNTVCT NERGWITYQG FLSQWTLTTY LDVQRCLEYL 360
GYLGYSILTE QESQASAVTV TRDKKIDLQK KQTQRNVFRC NVIGVKNCGK SGVLQALLGR 420
NLMRQKKIRE DHKSYYAINT VYVYGQEKYL LLHDISESEF LTEAEIICDV VCLVYDVSNP 480
KSFEYCARIF KQHFMDSRIP CLIVAAKSDL HEVKQEYSIS PTDFCRKHKM PPPQAFTCNT 540
ADAPSKDIFV KLTTMAMYPH VTQADLKSST FWLRASFGAT VFAVLGFAMY KALLKQR 597 
Gene Ontology
 GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
 GO:0005509; F:calcium ion binding; IEA:InterPro.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0003924; F:GTPase activity; IEA:InterPro.
 GO:0019725; P:cellular homeostasis; IEA:InterPro.
 GO:0006184; P:GTP catabolic process; IEA:GOC.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro. 
Interpro
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR013566; EF_hand_assoc_1.
 IPR013567; EF_hand_assoc_2.
 IPR002048; EF_hand_dom.
 IPR020860; MIRO.
 IPR013684; MIRO-like.
 IPR027417; P-loop_NTPase.
 IPR001806; Small_GTPase.
 IPR021181; Small_GTPase_Miro. 
Pfam
 PF08355; EF_assoc_1
 PF08356; EF_assoc_2
 PF13405; EF_hand_4
 PF08477; Miro
 PF00071; Ras 
SMART
 SM00054; EFh 
PROSITE
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2
 PS51423; MIRO 
PRINTS
 PR00449; RASTRNSFRMNG.