CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003167
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 HTH-type transcriptional regulator CysB 
Protein Synonyms/Alias
 Cys regulon transcriptional activator 
Gene Name
 cysB 
Gene Synonyms/Alias
 b1275; JW1267 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
35TSQPGISKQVRMLEDacetylation[1]
76IAREVLSKVDAIKSVacetylation[1]
81LSKVDAIKSVAGEHTacetylation[1]
92GEHTWPDKGSLYIATacetylation[1]
113YALPNVIKGFIERYPacetylation[1]
271IFSHSTTKIGFRRSTacetylation[1]
317EEIEVMFKDIKLPEKacetylation[1]
320EVMFKDIKLPEK***acetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 
Functional Description
 This protein is a positive regulator of gene expression for the cysteine regulon, a system of 10 or more loci involved in the biosynthesis of L-cysteine from inorganic sulfate. The inducer for CysB is N-acetylserine. CysB inhibits its own transcription. 
Sequence Annotation
 DOMAIN 1 59 HTH lysR-type.
 DNA_BIND 19 38 H-T-H motif (By similarity).  
Keyword
 Activator; Amino-acid biosynthesis; Complete proteome; Cysteine biosynthesis; Cytoplasm; DNA-binding; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 324 AA 
Protein Sequence
MKLQQLRYIV EVVNHNLNVS STAEGLYTSQ PGISKQVRML EDELGIQIFS RSGKHLTQVT 60
PAGQEIIRIA REVLSKVDAI KSVAGEHTWP DKGSLYIATT HTQARYALPN VIKGFIERYP 120
RVSLHMHQGS PTQIADAVSK GNADFAIATE ALHLYEDLVM LPCYHWNRAI VVTPDHPLAG 180
KKAITIEELA QYPLVTYTFG FTGRSELDTA FNRAGLTPRI VFTATDADVI KTYVRLGLGV 240
GVIASMAVDP VADPDLVRVD AHDIFSHSTT KIGFRRSTFL RSYMYDFIQR FAPHLTRDVV 300
DAAVALRSNE EIEVMFKDIK LPEK 324 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IDA:EcoCyc.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0019344; P:cysteine biosynthetic process; IEA:UniProtKB-KW.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IGI:EcoCyc.
 GO:0006351; P:transcription, DNA-dependent; IGI:EcoCyc. 
Interpro
 IPR005119; LysR_subst-bd.
 IPR000847; Tscrpt_reg_HTH_LysR.
 IPR011991; WHTH_DNA-bd_dom. 
Pfam
 PF00126; HTH_1
 PF03466; LysR_substrate 
SMART
  
PROSITE
 PS50931; HTH_LYSR 
PRINTS
 PR00039; HTHLYSR.