CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-030781
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Plastin-3 
Protein Synonyms/Alias
 cDNA FLJ59681, highly similar to Plastin-3 
Gene Name
 PLS3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30NMPLPGYKVREIIQKubiquitination[1]
37KVREIIQKLMLDGDRubiquitination[1]
69VKSSDIAKTFRKAINubiquitination[1]
78FRKAINRKEGICALGubiquitination[1, 2]
113AFVNWINKALENDPDubiquitination[1, 3]
136PNTDDLFKAVGDGIVubiquitination[2]
146GDGIVLCKMINLSVPubiquitination[1, 4]
244ETLEELMKLSPEELLubiquitination[2, 4]
287SNSVKDSKAYFHLLNubiquitination[1]
369NKYPALTKPENQDIDubiquitination[1, 2, 5]
424LQLYERIKVPVDWSKubiquitination[1]
434VDWSKVNKPPYPKLGubiquitination[3, 6]
446KLGANMKKLENCNYAacetylation[6]
446KLGANMKKLENCNYAubiquitination[3, 6, 7]
569GNLTEDDKHNNAKYAacetylation[8]
598PEDLVEVKPKMVMTVubiquitination[1, 4, 5]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224
Functional Description
  
Sequence Annotation
  
Keyword
 Actin-binding; Calcium; Complete proteome; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 617 AA 
Protein Sequence
MEDLNSNGFI CDYELHELFK EANMPLPGYK VREIIQKLML DGDRNKDGKI SFDEFVYIFQ 60
EVKSSDIAKT FRKAINRKEG ICALGGTSEL SSEGTQHSYS EEEKYAFVNW INKALENDPD 120
CRHVIPMNPN TDDLFKAVGD GIVLCKMINL SVPDTIDERA INKKKLTPFI IQENLNLALN 180
SASAIGCHVV NIGAEDLRAG KPHLVLGLLW QIIKIGLFAD IELSRNEALA ALLRDGETLE 240
ELMKLSPEEL LLRWANFHLE NSGWQKINNF SADIKLIDFS NSVKDSKAYF HLLNQIAPKG 300
QKEGEPRIDI NMSGFNETDD LKRAESMLQQ ADKLGCRQFV TPADVVSGNP KLNLAFVANL 360
FNKYPALTKP ENQDIDWTLL EGETREERTF RNWMNSLGVN PHVNHLYADL QDALVILQLY 420
ERIKVPVDWS KVNKPPYPKL GANMKKLENC NYAVELGKHP AKFSLVGIGG QDLNDGNQTL 480
TLALVWQLMR RYTLNVLEDL GDGQKANDDI IVNWVNRTLS EAGKSTSIQS FKDKTISSSL 540
AVVDLIDAIQ PGCINYDLVK SGNLTEDDKH NNAKYAVSMA RRIGARVYAL PEDLVEVKPK 600
MVMTVFACLM GRGMKRV 617 
Gene Ontology
 GO:0005509; F:calcium ion binding; IEA:InterPro. 
Interpro
 IPR001589; Actinin_actin-bd_CS.
 IPR001715; CH-domain.
 IPR011992; EF-hand-like_dom.
 IPR018247; EF_Hand_1_Ca_BS.
 IPR002048; EF_hand_dom. 
Pfam
 PF00307; CH
 PF13499; EF_hand_5 
SMART
 SM00033; CH 
PROSITE
 PS00019; ACTININ_1
 PS00020; ACTININ_2
 PS50021; CH
 PS00018; EF_HAND_1
 PS50222; EF_HAND_2 
PRINTS