CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018431
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cysteine--tRNA ligase 
Protein Synonyms/Alias
 Cysteinyl-tRNA synthetase; CysRS 
Gene Name
 cysS 
Gene Synonyms/Alias
 STM0537 
Created Date
 July 27, 2013 
Organism
 Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) 
NCBI Taxa ID
 99287 
Lysine Modification
Position
Peptide
Type
References
73NITDIDDKIIKRANEacetylation[1]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
  
Sequence Annotation
 MOTIF 30 40 "HIGH" region.
 MOTIF 266 270 "KMSKS" region.
 METAL 28 28 Zinc (By similarity).
 METAL 209 209 Zinc (By similarity).
 METAL 234 234 Zinc (By similarity).
 METAL 238 238 Zinc (By similarity).
 BINDING 269 269 ATP (By similarity).  
Keyword
 Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm; Ligase; Metal-binding; Nucleotide-binding; Protein biosynthesis; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 461 AA 
Protein Sequence
MLKIFNTLTR QKEEFKPIHA GEVGMYVCGI TVYDLCHIGH GRTFVAFDVV ARYLRFLGYK 60
LKYVRNITDI DDKIIKRANE NGESFVALVD RMIAEMHQDF DALNILRPDS EPRATHHIQE 120
IIELTRTLIE KGHAYVADNG DVMFDVPTDP TYGQLSRQDL EQLQAGARVD VVDVKRNPMD 180
FVLWKMSKEG EPSWPSPWGE GRPGWHIECS AMNCKQLGNH FDIHGGGSDL MFPHHENEIA 240
QSTCAHDGEY VNYWMHSGMV MVDREKMSKS LGNFFTVRDV LKYYDAETVR YFLMSGHYRS 300
QLNYSEENLK QARASLERLY TALRGTDKSA APAGGEAFEA RFVEAMNDDF NTPEAYSVLF 360
DMAREVNRLK GEDMTAANAM ASHLRKISGV LGLLEQEPDV FLQSGAQADD GEVAEIEALI 420
QQRLDARKAK DWAAADAARD RLAEMGIILE DGPQGTTWRR K 461 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004817; F:cysteine-tRNA ligase activity; IEA:HAMAP.
 GO:0008270; F:zinc ion binding; IEA:HAMAP.
 GO:0006423; P:cysteinyl-tRNA aminoacylation; IEA:HAMAP. 
Interpro
 IPR015803; Cys-tRNA-ligase.
 IPR015273; Cys-tRNA-synt_Ia_DALR.
 IPR024909; Cys-tRNA/MSH_ligase.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR009080; tRNAsynth_1a_anticodon-bd. 
Pfam
 PF09190; DALR_2
 PF01406; tRNA-synt_1e 
SMART
 SM00840; DALR_2 
PROSITE
 PS00178; AA_TRNA_LIGASE_I 
PRINTS
 PR00983; TRNASYNTHCYS.