| Tag | Content |
|---|
| CPLM ID | CPLM-005406 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Proline utilization trans-activator |
Protein Synonyms/Alias | |
Gene Name | PUT3 |
Gene Synonyms/Alias | YKL015W |
Created Date | July 27, 2013 |
Organism | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
NCBI Taxa ID | 559292 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 74 | KKIVVSTKYLQQLQK | acetylation | [1] |
|
Reference | [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae. Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C. Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [ PMID: 22865919] |
Functional Description | Positive activator of the proline utilization pathway. Binds to the promoters of PUT1 and PUT2 genes. Recognizes and binds to the DNA sequence 5'-CGG-N(10)-CCG-3'. |
Sequence Annotation | DNA_BIND 34 60 Zn(2)-C6 fungal-type.
METAL 34 34 Zinc 1.
METAL 34 34 Zinc 2.
METAL 37 37 Zinc 1.
METAL 44 44 Zinc 1.
METAL 50 50 Zinc 1.
METAL 50 50 Zinc 2.
METAL 53 53 Zinc 2.
METAL 60 60 Zinc 2. |
Keyword | 3D-structure; Activator; Complete proteome; DNA-binding; Metal-binding; Nucleus; Proline metabolism; Reference proteome; Transcription; Transcription regulation; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 979 AA |
Protein Sequence | MVTDQGSRHS IQSKQPAYVN KQPQKRQQRS SVACLSCRKR HIKCPGGNPC QKCVTSNAIC 60
EYLEPSKKIV VSTKYLQQLQ KDLNDKTEEN NRLKALLLER PVSVRGKDNS DDDERHINNA 120
PSSDTLEVSS APAAPIFDLM SNSNTASDND NDDDNSNRIT NNRSYDHSLE KYYKKAISIF 180
KQPANANGEN GNGANGHEDD DEDDEEISTN FAQRSGRLIE SHNGFHYFVG SSSMTLFGLE 240
IQSLVTKYIS VKNFRPLPIN TKNKILNSNL NPAISSFINS NNYLFSSYNF LNPISTIVNL 300
NSINDNLSPL MFKIILKSDT DGSSGQEEVI QFQLPSYNYT KLLIDCFINY NDGCFYFFNE 360
GLVKCGINKL YLENKWLYYD NTKKALDNEN DPILQAVWFC KILLILAVGE MYLGSINNEM 420
LKNYSNQPKL PGSKFFQMGS KIFNCLFSSE RLENVTKKGG IEVLLLYAFF LQVADYTLAS 480
YFYFGQALRT CLILGLHVDS QSDTLSRYEI EHHRRLWWTV YMFERMLSSK AGLPLSFTDY 540
TISTALPADI DDETIEEKNS HYVFRKAELI SNCVTIVKIN AQILSKLYQR QPETNIIITL 600
KVVIKQLLEW RNNLSDSLQV DFTQKDEDFK ISRLSTNMFT EYFQGINLAV RPLLFHFASI 660
QLKRFKTSNT FVNLQNYSAT ISSLLTCSLH ASVNTIRSLW SLLQNSMLAM FSYMDREYLF 720
TSSCTLLLFN TAFGIHEQTL YHLDHSLEIF TQMRNLGNIP AGLRRAQLLT LMANLDFHGI 780
MNDLITKYND ILKFDSMNCE NDNIVEDSNE PKRETEKCKP HKDGDRIDPS IIDCDKSNTN 840
TNMIKNESIS NIVSILPEGA KPTLTDYSNG NNDVNDINVN NSEPSTFFDI ITASLENSYQ 900
TTLTEKGSQV MEKNMDQLDS VHNLNDDDLQ QLLEDLGNID HSDEKLWKEI TDQAMWLGNT 960
MDPTAAAGSE IDFTDYLGP 979 |
Gene Ontology | GO:0005634; C:nucleus; IC:SGD. GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:SGD. GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IMP:SGD. GO:0008270; F:zinc ion binding; IEA:InterPro. GO:2001158; P:positive regulation of proline catabolic process to glutamate; IMP:SGD. GO:0006560; P:proline metabolic process; IEA:UniProtKB-KW. GO:0000972; P:transcription-dependent tethering of RNA polymerase II gene DNA at nuclear periphery; IMP:SGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |