CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008994
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial 
Protein Synonyms/Alias
 3-oxoacid CoA-transferase 1; Somatic-type succinyl-CoA:3-oxoacid CoA-transferase; SCOT-s 
Gene Name
 OXCT1 
Gene Synonyms/Alias
 OXCT; SCOT 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
173QEGGSPIKYNKDGSVacetylation[1]
173QEGGSPIKYNKDGSVubiquitination[2]
185GSVAIASKPREVREFacetylation[3]
185GSVAIASKPREVREFubiquitination[2, 4]
421MIPGKMVKGMGGAMDubiquitination[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 
Functional Description
 Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate. 
Sequence Annotation
 ACT_SITE 344 344 Acyl-thioester intermediate (By
 MOD_RES 170 170 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Direct protein sequencing; Disease mutation; Mitochondrion; Phosphoprotein; Polymorphism; Reference proteome; Transferase; Transit peptide. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 520 AA 
Protein Sequence
MAALKLLSSG LRLCASARGS GATWYKGCVC SFSTSAHRHT KFYTDPVEAV KDIPDGATVL 60
VGGFGLCGIP ENLIDALLKT GVKGLTAVSN NAGVDNFGLG LLLRSKQIKR MVSSYVGENA 120
EFERQYLSGE LEVELTPQGT LAERIRAGGA GVPAFYTPTG YGTLVQEGGS PIKYNKDGSV 180
AIASKPREVR EFNGQHFILE EAITGDFALV KAWKADRAGN VIFRKSARNF NLPMCKAAET 240
TVVEVEEIVD IGAFAPEDIH IPQIYVHRLI KGEKYEKRIE RLSIRKEGDG EAKSAKPGDD 300
VRERIIKRAA LEFEDGMYAN LGIGIPLLAS NFISPNITVH LQSENGVLGL GPYPRQHEAD 360
ADLINAGKET VTILPGASFF SSDESFAMIR GGHVDLTMLG AMQVSKYGDL ANWMIPGKMV 420
KGMGGAMDLV SSAKTKVVVT MEHSAKGNAH KIMEKCTLPL TGKQCVNRII TEKAVFDVDK 480
KKGLTLIELW EGLTVDDVQK STGCDFAVSP KLMPMQQIAN 520 
Gene Ontology
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0008260; F:3-oxoacid CoA-transferase activity; IMP:UniProtKB.
 GO:0042803; F:protein homodimerization activity; NAS:UniProtKB.
 GO:0060612; P:adipose tissue development; IEA:Compara.
 GO:0007420; P:brain development; IEA:Compara.
 GO:0044255; P:cellular lipid metabolic process; TAS:Reactome.
 GO:0071229; P:cellular response to acid; IEA:Compara.
 GO:0071333; P:cellular response to glucose stimulus; IEA:Compara.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0046952; P:ketone body catabolic process; TAS:Reactome.
 GO:0042182; P:ketone catabolic process; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0014823; P:response to activity; IEA:Compara.
 GO:0042493; P:response to drug; IEA:Compara.
 GO:0045471; P:response to ethanol; IEA:Compara.
 GO:0009725; P:response to hormone stimulus; IEA:Compara.
 GO:0007584; P:response to nutrient; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara. 
Interpro
 IPR012792; 3-oxoacid_CoA-transf_A.
 IPR012791; 3-oxoacid_CoA-transf_B.
 IPR014388; 3-oxoacid_CoA-transferase.
 IPR004165; CoA_trans_fam_I.
 IPR004164; CoA_transf_AS.
 IPR004163; CoA_transf_BS. 
Pfam
 PF01144; CoA_trans 
SMART
 SM00882; CoA_trans 
PROSITE
 PS01273; COA_TRANSF_1
 PS01274; COA_TRANSF_2 
PRINTS