CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-016339
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional repressor p66-alpha 
Protein Synonyms/Alias
 Hp66alpha; GATA zinc finger domain-containing protein 2A 
Gene Name
 GATAD2A 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
30DVESKKIKMERGLLAsumoylation[1]
464RLKAAFVKALQQEQEubiquitination[2, 3]
487GTAPAQAKAEPTAAPsumoylation[1]
Reference
 [1] SUMO modification enhances p66-mediated transcriptional repression of the Mi-2/NuRD complex.
 Gong Z, Brackertz M, Renkawitz R.
 Mol Cell Biol. 2006 Jun;26(12):4519-28. [PMID: 16738318]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Transcriptional repressor. Enhances MBD2-mediated repression. Efficient repression requires the presence of GATAD2B. 
Sequence Annotation
 ZN_FING 411 464 GATA-type.
 REGION 144 178 CR1; MBD2-binding.
 REGION 340 480 CR2; histone tail-binding.
 MOD_RES 49 49 Phosphothreonine.
 MOD_RES 100 100 Phosphoserine.
 MOD_RES 107 107 Phosphoserine.
 MOD_RES 114 114 Phosphoserine.
 MOD_RES 189 189 Phosphothreonine.
 MOD_RES 340 340 Phosphoserine.
 MOD_RES 343 343 Phosphoserine.
 MOD_RES 546 546 Phosphoserine.
 MOD_RES 548 548 Phosphoserine.
 MOD_RES 598 598 Phosphoserine.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repressor; Transcription; Transcription regulation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 633 AA 
Protein Sequence
MTEEACRTRS QKRALERDPT EDDVESKKIK MERGLLASDL NTDGDMRVTP EPGAGPTQGL 60
LRATEATAMA MGRGEGLVGD GPVDMRTSHS DMKSERRPPS PDVIVLSDNE QPSSPRVNGL 120
TTVALKETST EALMKSSPEE RERMIKQLKE ELRLEEAKLV LLKKLRQSQI QKEATAQKPT 180
GSVGSTVTTP PPLVRGTQNI PAGKPSLQTS SARMPGSVIP PPLVRGGQQA SSKLGPQASS 240
QVVMPPLVRG AQQIHSIRQH SSTGPPPLLL APRASVPSVQ IQGQRIIQQG LIRVANVPNT 300
SLLVNIPQPT PASLKGTTAT SAQANSTPTS VASVVTSAES PASRQAAAKL ALRKQLEKTL 360
LEIPPPKPPA PEMNFLPSAA NNEFIYLVGL EEVVQNLLET QGRMSAATVL SREPYMCAQC 420
KTDFTCRWRE EKSGAIMCEN CMTTNQKKAL KVEHTSRLKA AFVKALQQEQ EIEQRLLQQG 480
TAPAQAKAEP TAAPHPVLKQ VIKPRRKLAF RSGEARDWSN GAVLQASSQL SRGSATTPRG 540
VLHTFSPSPK LQNSASATAL VSRTGRHSER TVSAGKGSAT SNWKKTPLST GGTLAFVSPS 600
LAVHKSSSAV DRQREYLLDM IPPRSIPQSA TWK 633 
Gene Ontology
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0016581; C:NuRD complex; IDA:UniProtKB.
 GO:0030674; F:protein binding, bridging; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0021506; P:anterior neuropore closure; IEA:Compara.
 GO:0001568; P:blood vessel development; IEA:Compara.
 GO:0006306; P:DNA methylation; TAS:UniProtKB.
 GO:0010172; P:embryonic body morphogenesis; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0045892; P:negative regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0001842; P:neural fold formation; IEA:Compara.
 GO:0012501; P:programmed cell death; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR000679; Znf_GATA.
 IPR013088; Znf_NHR/GATA. 
Pfam
 PF00320; GATA 
SMART
  
PROSITE
 PS00344; GATA_ZN_FINGER_1
 PS50114; GATA_ZN_FINGER_2 
PRINTS