CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008255
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Guanine nucleotide-binding protein G(q) subunit alpha 
Protein Synonyms/Alias
 Guanine nucleotide-binding protein alpha-q 
Gene Name
 GNAQ 
Gene Synonyms/Alias
 GAQ 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
16CCLSEEAKEARRINDubiquitination[1, 2]
52LGTGESGKSTFIKQMubiquitination[1]
102DTLKIPYKYEHNKAHacetylation[3]
102DTLKIPYKYEHNKAHubiquitination[1, 4]
120VREVDVEKVSAFENPubiquitination[1, 4]
158YQLSDSTKYYLNDLDubiquitination[1, 4]
252ENRMEESKALFRTIIubiquitination[1, 5]
354TILQLNLKEYNLV**ubiquitination[5]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro) (By similarity). 
Sequence Annotation
 NP_BIND 46 53 GTP (By similarity).
 NP_BIND 180 186 GTP (By similarity).
 NP_BIND 205 209 GTP (By similarity).
 NP_BIND 274 277 GTP (By similarity).
 METAL 53 53 Magnesium (By similarity).
 METAL 186 186 Magnesium (By similarity).
 BINDING 331 331 GTP; via amide nitrogen (By similarity).
 MOD_RES 183 183 ADP-ribosylarginine; by cholera toxin (By
 LIPID 9 9 S-palmitoyl cysteine (By similarity).
 LIPID 10 10 S-palmitoyl cysteine (By similarity).  
Keyword
 ADP-ribosylation; Complete proteome; GTP-binding; Lipoprotein; Magnesium; Membrane; Metal-binding; Nucleotide-binding; Nucleus; Palmitate; Polymorphism; Reference proteome; Transducer. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 359 AA 
Protein Sequence
MTLESIMACC LSEEAKEARR INDEIERQLR RDKRDARREL KLLLLGTGES GKSTFIKQMR 60
IIHGSGYSDE DKRGFTKLVY QNIFTAMQAM IRAMDTLKIP YKYEHNKAHA QLVREVDVEK 120
VSAFENPYVD AIKSLWNDPG IQECYDRRRE YQLSDSTKYY LNDLDRVADP AYLPTQQDVL 180
RVRVPTTGII EYPFDLQSVI FRMVDVGGQR SERRKWIHCF ENVTSIMFLV ALSEYDQVLV 240
ESDNENRMEE SKALFRTIIT YPWFQNSSVI LFLNKKDLLE EKIMYSHLVD YFPEYDGPQR 300
DAQAAREFIL KMFVDLNPDS DKIIYSHFTC ATDTENIRFV FAAVKDTILQ LNLKEYNLV 359 
Gene Ontology
 GO:0005737; C:cytoplasm; TAS:ProtInc.
 GO:0005834; C:heterotrimeric G-protein complex; IBA:RefGenome.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:RefGenome.
 GO:0001664; F:G-protein coupled receptor binding; IBA:RefGenome.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0005096; F:GTPase activator activity; IDA:UniProtKB.
 GO:0003924; F:GTPase activity; TAS:ProtInc.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004871; F:signal transducer activity; IBA:RefGenome.
 GO:0007202; P:activation of phospholipase C activity; TAS:ProtInc.
 GO:0007189; P:adenylate cyclase-activating G-protein coupled receptor signaling pathway; IBA:RefGenome.
 GO:0007610; P:behavior; IEA:Compara.
 GO:0048066; P:developmental pigmentation; IEA:Compara.
 GO:0042733; P:embryonic digit morphogenesis; IEA:Compara.
 GO:0021884; P:forebrain neuron development; IEA:Compara.
 GO:0007215; P:glutamate receptor signaling pathway; IBA:RefGenome.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0006469; P:negative regulation of protein kinase activity; IMP:BHF-UCL.
 GO:0016322; P:neuron remodeling; IEA:Compara.
 GO:0060158; P:phospholipase C-activating dopamine receptor signaling pathway; IBA:RefGenome.
 GO:0030168; P:platelet activation; TAS:Reactome.
 GO:0009791; P:post-embryonic development; IEA:Compara.
 GO:0050821; P:protein stabilization; IMP:BHF-UCL.
 GO:0001508; P:regulation of action potential; IBA:RefGenome.
 GO:0035412; P:regulation of catenin import into nucleus; IMP:BHF-UCL.
 GO:0045634; P:regulation of melanocyte differentiation; IEA:Compara.
 GO:0001501; P:skeletal system development; IEA:Compara. 
Interpro
 IPR000654; Gprotein_alpha_Q.
 IPR001019; Gprotein_alpha_su.
 IPR011025; GproteinA_insert.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00503; G-alpha 
SMART
 SM00275; G_alpha 
PROSITE
  
PRINTS
 PR00318; GPROTEINA.
 PR00442; GPROTEINAQ.