UniProt Accession

 PICK1_HUMAN; Q9NRD5; O95906 

Genbank Protein ID

 AB026491; AF231710; AL049654; CR456550; AL031587; BC017561 

Genbank Nucleotide ID

 BAA89294.1; AAF97502.1; CAB41082.1; CAG30436.1; CAB37478.1; AAH17561.1 

Protein Name

 PRKCA-binding protein 

Protein Synonyms/Alias

 Protein interacting with C kinase 1; Protein kinase C-alpha-binding protein 

Gene Name

 PICK1 

Gene Synonyms/Alias

 PRKCABP 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
151	LCNDGLVKRLEELER	ubiquitination	[1]
209	AASEAFVKFADAHRS	ubiquitination	[2, 3]
408	GAAGPLDKGGSWCDS	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Probable adapter protein that bind to and organize the subcellular localization of a variety of membrane proteins containing some PDZ recognition sequence. Involved in the clustering of various receptors, possibly by acting at the receptor internalization level. Plays a role in synaptic plasticity by regulating the trafficking and internalization of AMPA receptors. May be regulated upon PRKCA activation. May regulate heteromeric ASIC1/ASIC3 channel. 

Sequence Annotation

 DOMAIN 22 105 PDZ.
 DOMAIN 144 357 AH.
 METAL 44 44 Zinc (By similarity).
 METAL 46 46 Zinc (By similarity).
 MOD_RES 82 82 Phosphothreonine.  

Keyword

 3D-structure; Calcium; Cell junction; Complete proteome; Cytoplasm; Metal-binding; Phosphoprotein; Reference proteome; Synapse; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 415 AA 

Protein Sequence

Gene Ontology

 GO:0030054; C:cell junction; IEA:UniProtKB-KW.
 GO:0030666; C:endocytic vesicle membrane; TAS:Reactome.
 GO:0005794; C:Golgi apparatus; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0048471; C:perinuclear region of cytoplasm; ISS:UniProtKB.
 GO:0005886; C:plasma membrane; IDA:UniProtKB.
 GO:0042734; C:presynaptic membrane; IDA:UniProtKB.
 GO:0016887; F:ATPase activity; ISS:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0005080; F:protein kinase C binding; ISS:UniProtKB.
 GO:0005102; F:receptor binding; ISS:UniProtKB.
 GO:0043045; P:DNA methylation involved in embryo development; NAS:UniProtKB.
 GO:0043046; P:DNA methylation involved in gamete generation; TAS:UniProtKB.
 GO:0015844; P:monoamine transport; IDA:UniProtKB.
 GO:0045161; P:neuronal ion channel clustering; TAS:UniProtKB.
 GO:0007205; P:protein kinase C-activating G-protein coupled receptor signaling pathway; IDA:UniProtKB.
 GO:0006468; P:protein phosphorylation; ISS:UniProtKB.
 GO:0006605; P:protein targeting; IEA:Compara.
 GO:0043113; P:receptor clustering; ISS:UniProtKB.
 GO:0006890; P:retrograde vesicle-mediated transport, Golgi to ER; NAS:UniProtKB.
 GO:0007268; P:synaptic transmission; TAS:Reactome. 

Interpro

 IPR027267; AH/BAR-dom.
 IPR010504; AH_dom.
 IPR001478; PDZ. 

Pfam

 PF06456; Arfaptin
 PF00595; PDZ 

SMART

 SM01015; Arfaptin
 SM00228; PDZ 

PROSITE

 PS50870; AH
 PS50106; PDZ 

PRINTS