CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024173
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Heterogeneous nuclear ribonucleoprotein F 
Protein Synonyms/Alias
 hnRNP F; Heterogeneous nuclear ribonucleoprotein F, N-terminally processed 
Gene Name
 Hnrnpf 
Gene Synonyms/Alias
 Hnrpf 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
87HRYIEVFKSHRTEMDubiquitination[1]
98TEMDWVLKHSGPNSAubiquitination[1]
167ASQELAEKALGKHKEubiquitination[1]
185HRYIEVFKSSQEEVRubiquitination[1]
200SYSDPPLKFMSVQRPubiquitination[1]
224RRYIGIVKQAGLDRMubiquitination[1]
299HMRGLPYKATENDIYubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state (By similarity). 
Sequence Annotation
 DOMAIN 11 90 RRM 1.
 DOMAIN 111 188 RRM 2.
 DOMAIN 289 366 RRM 3.
 REGION 81 86 Interaction with RNA (By similarity).
 REGION 179 184 Interaction with RNA (By similarity).
 REGION 355 360 Interaction with RNA (By similarity).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 2 2 N-acetylmethionine; in Heterogeneous
 MOD_RES 104 104 Phosphoserine (By similarity).
 MOD_RES 161 161 Phosphoserine (By similarity).
 MOD_RES 187 187 Phosphoserine (By similarity).
 MOD_RES 224 224 N6-acetyllysine (By similarity).
 CROSSLNK 72 72 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Alternative splicing; Complete proteome; Isopeptide bond; mRNA processing; mRNA splicing; Nucleus; Phosphoprotein; Reference proteome; Repeat; Ribonucleoprotein; RNA-binding; Spliceosome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 415 AA 
Protein Sequence
MMLGPEGGEG YVVKLRGLPW SCSIEDVQNF LSDCTIHDGV AGVHFIYTRE GRQSGEAFVE 60
LESEDDVKLA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS GPNSADSAND GFVRLRGLPF 120
GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK ITGEAFVQFA SQELAEKALG KHKERIGHRY 180
IEVFKSSQEE VRSYSDPPLK FMSVQRPGPY DRPGTARRYI GIVKQAGLDR MRSGAYSAGY 240
GGYEEYSGLS DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA 300
TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA NMQHRYIELF 360
LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC YGAGYSGQNS MGGYD 415 
Gene Ontology
 GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
 GO:0005681; C:spliceosomal complex; IEA:UniProtKB-KW.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0003727; F:single-stranded RNA binding; ISS:UniProtKB.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0043484; P:regulation of RNA splicing; ISS:UniProtKB.
 GO:0008380; P:RNA splicing; IEA:UniProtKB-KW. 
Interpro
 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR012996; Znf_CHHC. 
Pfam
 PF08080; zf-RNPHF 
SMART
 SM00360; RRM 
PROSITE
 PS50102; RRM 
PRINTS