UniProt Accession

 FBX9_HUMAN; Q9UK97; A6NFW3; B3KMM6; O75986; Q59EH8; Q6PKH7; Q9NT57; Q9Y593 

Genbank Protein ID

 AF176704; AY858798; AL137520; AK021559; AB209833; AL031178; AL031178; BC000650; AF174597 

Genbank Nucleotide ID

 AAF03704.1; AAW51115.1; CAB70786.2; BAG51038.1; BAD93070.1; CAI20262.1; CAI20263.1; AAH00650.2; AAF04518.1 

Protein Name

 F-box only protein 9 

Protein Synonyms/Alias

 Cross-immune reaction antigen 1; Renal carcinoma antigen NY-REN-57 

Gene Name

 FBXO9 

Gene Synonyms/Alias

 FBX9; VCIA1 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
115	GALYEAIKFYRRAMQ	ubiquitination	[1, 2]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Substrate recognition component of a (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of TTI1 and TELO2 in a CK2-dependent manner, thereby directly regulating mTOR signaling. SCF(FBXO9) recognizes and binds mTORC1-bound TTI1 and TELO2 when they are phosphorylated by CK2 following growth factor deprivation, leading to their degradation. In contrast, the SCF(FBXO9) does not mediate ubiquitination of TTI1 and TELO2 when they are part of the mTORC2 complex. As a consequence, mTORC1 is inactivated to restrain cell growth and protein translation, while mTORC2 is activated due to the relief of feedback inhibition by mTORC1. 

Sequence Annotation

 REPEAT 94 127 TPR.
 DOMAIN 185 236 F-box.
 MOD_RES 136 136 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Phosphoprotein; Reference proteome; TPR repeat; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 447 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0019005; C:SCF ubiquitin ligase complex; IDA:UniProtKB.
 GO:0004842; F:ubiquitin-protein ligase activity; NAS:UniProtKB.
 GO:0032006; P:regulation of TOR signaling cascade; IMP:UniProtKB.
 GO:0031146; P:SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; IDA:UniProtKB. 

Interpro

 IPR001810; F-box_dom_cyclin-like. 

Pfam

 PF00646; F-box 

SMART

  

PROSITE

 PS50181; FBOX
 PS50005; TPR
 PS50293; TPR_REGION 

PRINTS