CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-011090
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Antigen peptide transporter 2 
Protein Synonyms/Alias
 APT2; ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein 
Gene Name
 TAP2 
Gene Synonyms/Alias
 ABCB3; PSF2; RING11; Y1 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
245LGFFQETKTGELNSRubiquitination[1]
603IYTDVGEKGSQLAAGubiquitination[1]
612SQLAAGQKQRLAIARubiquitination[1]
Reference
 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 
Functional Description
 Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. 
Sequence Annotation
 DOMAIN 152 435 ABC transmembrane type-1.
 DOMAIN 468 686 ABC transporter.
 NP_BIND 503 510 ATP (Potential).
 REGION 301 389 Involved in peptide-binding site.
 REGION 414 433 Involved in peptide-binding site.  
Keyword
 Adaptive immunity; Alternative splicing; ATP-binding; Complete proteome; Endoplasmic reticulum; Host-virus interaction; Immunity; Membrane; Nucleotide-binding; Peptide transport; Polymorphism; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 686 AA 
Protein Sequence
MRLPDLRPWT SLLLVDAALL WLLQGPLGTL LPQGLPGLWL EGTLRLGGLW GLLKLRGLLG 60
FVGTLLLPLC LATPLTVSLR ALVAGASRAP PARVASAPWS WLLVGYGAAG LSWSLWAVLS 120
PPGAQEKEQD QVNNKVLMWR LLKLSRPDLP LLVAAFFFLV LAVLGETLIP HYSGRVIDIL 180
GGDFDPHAFA SAIFFMCLFS FGSSLSAGCR GGCFTYTMSR INLRIREQLF SSLLRQDLGF 240
FQETKTGELN SRLSSDTTLM SNWLPLNANV LLRSLVKVVG LYGFMLSISP RLTLLSLLHM 300
PFTIAAEKVY NTRHQEVLRE IQDAVARAGQ VVREAVGGLQ TVRSFGAEEH EVCRYKEALE 360
QCRQLYWRRD LERALYLLVR RVLHLGVQML MLSCGLQQMQ DGELTQGSLL SFMIYQESVG 420
SYVQTLVYIY GDMLSNVGAA EKVFSYMDRQ PNLPSPGTLA PTTLQGVVKF QDVSFAYPNR 480
PDRPVLKGLT FTLRPGEVTA LVGPNGSGKS TVAALLQNLY QPTGGQVLLD EKPISQYEHC 540
YLHSQVVSVG QEPVLFSGSV RNNIAYGLQS CEDDKVMAAA QAAHADDFIQ EMEHGIYTDV 600
GEKGSQLAAG QKQRLAIARA LVRDPRVLIL DEATSALDVQ CEQAKTLWKF MIF 653 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005886; C:plasma membrane; IBA:RefGenome.
 GO:0042825; C:TAP complex; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IDA:UniProtKB.
 GO:0042288; F:MHC class I protein binding; IBA:RefGenome.
 GO:0015421; F:oligopeptide-transporting ATPase activity; IBA:RefGenome.
 GO:0042605; F:peptide antigen binding; IBA:RefGenome.
 GO:0015433; F:peptide antigen-transporting ATPase activity; IMP:UniProtKB.
 GO:0046978; F:TAP1 binding; IBA:RefGenome.
 GO:0046979; F:TAP2 binding; IBA:RefGenome.
 GO:0046980; F:tapasin binding; ISS:UniProtKB.
 GO:0002485; P:antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; IBA:RefGenome.
 GO:0002489; P:antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; IMP:UniProtKB.
 GO:0002479; P:antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; TAS:Reactome.
 GO:0002481; P:antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent; IBA:RefGenome.
 GO:0046967; P:cytosol to ER transport; IMP:UniProtKB.
 GO:0019060; P:intracellular transport of viral proteins in host cell; IMP:UniProtKB.
 GO:0046968; P:peptide antigen transport; IDA:UniProtKB.
 GO:0002591; P:positive regulation of antigen processing and presentation of peptide antigen via MHC class I; IBA:RefGenome.
 GO:0001916; P:positive regulation of T cell mediated cytotoxicity; IBA:RefGenome. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR013306; ABC_B3.
 IPR003439; ABC_transporter-like.
 IPR017871; ABC_transporter_CS.
 IPR017940; ABC_transporter_type1.
 IPR001140; ABC_transptr_TM_dom.
 IPR011527; ABC_transptrTM_dom_typ1.
 IPR005293; Ag_transporter2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00664; ABC_membrane
 PF00005; ABC_tran 
SMART
 SM00382; AAA 
PROSITE
 PS50929; ABC_TM1F
 PS00211; ABC_TRANSPORTER_1
 PS50893; ABC_TRANSPORTER_2 
PRINTS
 PR01897; TAP2PROTEIN.