CPLM-023604

Genbank Nucleotide ID

Protein Synonyms/Alias

Endo-glucoronidase; Heparanase-1; Hpa1; Heparanase 8 kDa subunit; Heparanase 50 kDa subunit

HEP; HPA; HPA1; HPR1; HPSE1; HSE1

Homo sapiens (Human)

Position	Peptide	Type	References
98	YLRFGGTKTDFLIFD	acetylation	[1]

[1] Regulation of cellular metabolism by protein lysine acetylation.
Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]

Functional Description

Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis.

REGION 158 162 Heparin/HS-binding.
REGION 270 280 Heparin/HS-binding.
REGION 527 543 Required for transferring proheparanase
ACT_SITE 225 225 Proton donor (Potential).
ACT_SITE 343 343 Nucleophile (Potential).
CARBOHYD 162 162 N-linked (GlcNAc...).
CARBOHYD 178 178 N-linked (GlcNAc...).
CARBOHYD 200 200 N-linked (GlcNAc...).
CARBOHYD 217 217 N-linked (GlcNAc...).
CARBOHYD 238 238 N-linked (GlcNAc...).
CARBOHYD 459 459 N-linked (GlcNAc...).

Alternative splicing; Calcium; Cell adhesion; Complete proteome; Direct protein sequencing; Glycoprotein; Hydrolase; Lysosome; Magnesium; Membrane; Nucleus; Polymorphism; Reference proteome; Secreted; Signal.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
GO:0043202; C:lysosomal lumen; TAS:Reactome.
GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
GO:0045121; C:membrane raft; IEA:Compara.
GO:0005634; C:nucleus; IDA:UniProtKB.
GO:0004566; F:beta-glucuronidase activity; TAS:ProtInc.
GO:0030305; F:heparanase activity; IDA:UniProtKB.
GO:0046983; F:protein dimerization activity; IDA:UniProtKB.
GO:0045545; F:syndecan binding; IDA:UniProtKB.
GO:0005975; P:carbohydrate metabolic process; TAS:Reactome.
GO:0007160; P:cell-matrix adhesion; IDA:UniProtKB.
GO:0006027; P:glycosaminoglycan catabolic process; TAS:Reactome.
GO:0030200; P:heparan sulfate proteoglycan catabolic process; IDA:UniProtKB.
GO:0030194; P:positive regulation of blood coagulation; IDA:UniProtKB.
GO:0051798; P:positive regulation of hair follicle development; IEA:Compara.
GO:0033690; P:positive regulation of osteoblast proliferation; IDA:UniProtKB.
GO:0051897; P:positive regulation of protein kinase B signaling cascade; IDA:UniProtKB.
GO:0010575; P:positive regulation vascular endothelial growth factor production; IDA:UniProtKB.
GO:0051797; P:regulation of hair follicle development; IDA:UniProtKB.
GO:0061042; P:vascular wound healing; IEA:Compara.

IPR005199; Glyco_hydro_79.
IPR013781; Glyco_hydro_catalytic_dom.
IPR017853; Glycoside_hydrolase_SF.

PF03662; Glyco_hydro_79n