CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009946
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 
Protein Synonyms/Alias
 6PF-2-K/Fru-2,6-P2ase 1; PFK/FBPase 1; 6PF-2-K/Fru-2,6-P2ase liver isozyme; 6-phosphofructo-2-kinase; Fructose-2,6-bisphosphatase 
Gene Name
 Pfkfb1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
15LTQTRLQKIWIPHSSubiquitination[1]
114QCALAALKDVHKYLSubiquitination[1]
148SLILQFAKEHGYKVFubiquitination[1]
281SGLSARGKQYAYALAubiquitination[1]
307KVWTSHMKRTIQTAEubiquitination[1]
364KYRYRYPKGESYEDLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Synthesis and degradation of fructose 2,6-bisphosphate. 
Sequence Annotation
 NP_BIND 49 56 ATP (Potential).
 REGION 2 250 6-phosphofructo-2-kinase (By similarity).
 REGION 251 471 Fructose-2,6-bisphosphatase (By
 ACT_SITE 131 131 Potential.
 ACT_SITE 161 161 Potential.
 ACT_SITE 259 259 Tele-phosphohistidine intermediate (By
 ACT_SITE 328 328 Potential.
 ACT_SITE 393 393 Proton donor (By similarity).
 BINDING 105 105 Fructose-6-phosphate (By similarity).
 BINDING 196 196 Fructose-6-phosphate (By similarity).
 MOD_RES 2 2 N-acetylserine (By similarity).
 MOD_RES 33 33 Phosphoserine; by PKA (By similarity).  
Keyword
 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Hydrolase; Kinase; Multifunctional enzyme; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 471 AA 
Protein Sequence
MSREMGELTQ TRLQKIWIPH SSSSSLLQRR RGSSIPQFTN SPTMVIMVGL PARGKTYIST 60
KLTRYLNWIG TPTKVFNLGQ YRREAVSYRN YEFFRPDNME AQLIRKQCAL AALKDVHKYL 120
SREEGHVAVF DATNTTRERR SLILQFAKEH GYKVFFIESI CNDPDIIAEN IKQVKLGSPD 180
YIDCDQEKVL EDFLKRIECY EINYQPLDEE LDSHLSYIKI FDVGTRYMVN RVQDHVQSRT 240
AYYLMNIHVT PRSIYLCRHG ESELNLRGRI GGDSGLSARG KQYAYALANF IRSQSISSLK 300
VWTSHMKRTI QTAEALGVPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY 360
RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH 420
TVLKLTPVAY GCRVESIYLN VEAVNTHRDK PENVDITREP EEALDTVPAH Y 471 
Gene Ontology
 GO:0043540; C:6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 complex; ISS:UniProtKB.
 GO:0003873; F:6-phosphofructo-2-kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004331; F:fructose-2,6-bisphosphate 2-phosphatase activity; ISS:UniProtKB.
 GO:0070095; F:fructose-6-phosphate binding; IEA:Compara.
 GO:0006003; P:fructose 2,6-bisphosphate metabolic process; ISS:UniProtKB.
 GO:0006000; P:fructose metabolic process; IEA:InterPro.
 GO:0031100; P:organ regeneration; IEA:Compara.
 GO:0033133; P:positive regulation of glucokinase activity; IEA:Compara.
 GO:0051591; P:response to cAMP; IEA:Compara.
 GO:0033762; P:response to glucagon stimulus; IEA:Compara.
 GO:0051384; P:response to glucocorticoid stimulus; IEA:Compara.
 GO:0032868; P:response to insulin stimulus; IEA:Compara.
 GO:0042594; P:response to starvation; IEA:Compara. 
Interpro
 IPR003094; 6Pfruct_kin.
 IPR013079; 6Phosfructo_kin.
 IPR016260; Bifunct_6PFK/fruc_bisP_Ptase.
 IPR013078; His_Pase_superF_clade-1.
 IPR027417; P-loop_NTPase.
 IPR001345; PG/BPGM_mutase_AS. 
Pfam
 PF01591; 6PF2K
 PF00300; His_Phos_1 
SMART
 SM00855; PGAM 
PROSITE
 PS00175; PG_MUTASE 
PRINTS
 PR00991; 6PFRUCTKNASE.