CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019260
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like 
Protein Synonyms/Alias
 tRNA-dihydrouridine synthase 3-like 
Gene Name
 DUS3L 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
73ELAEPEAKRIRLEDGubiquitination[1, 2]
101GEQLQTQKRARGQNKubiquitination[1]
134LIQESAAKCFFGDRCubiquitination[2, 3, 4]
154VGRYLETKPADLGPRubiquitination[1, 2, 3, 4]
212SIRNGLDKALQQQLRubiquitination[2]
306KRLDIRGKLYLAPLTubiquitination[2, 4]
404PIDLVYKKGGGCALMubiquitination[2]
416ALMNRSTKFQQIVRGubiquitination[1, 2, 5, 6]
435LDVPLTVKIRTGVQEubiquitination[1, 2, 5, 6, 7]
526IARGALLKPWLFTEIubiquitination[1, 2, 3, 4, 5, 6]
534PWLFTEIKEQRHWDIubiquitination[2, 3]
619ETLMASQKAADWIRIubiquitination[1, 2]
643PSFAFLPKHKANAYKubiquitination[2]
645FAFLPKHKANAYK**ubiquitination[2]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (By similarity). 
Sequence Annotation
 ZN_FING 118 148 C3H1-type 1.
 ZN_FING 156 186 C3H1-type 2.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 236 236 Phosphoserine.
 MOD_RES 273 273 Phosphothreonine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; FAD; Flavoprotein; Metal-binding; Oxidoreductase; Phosphoprotein; Polymorphism; Reference proteome; Repeat; tRNA processing; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 650 AA 
Protein Sequence
MAEGTAEAPL ENGGGGDSGA GALERGVAPI KRQYLTTKEQ FHQFLEAKGQ EKTCRETEVG 60
DPAGNELAEP EAKRIRLEDG QTADGQTEEA AEPGEQLQTQ KRARGQNKGR PHVKPTNYDK 120
NRLCPSLIQE SAAKCFFGDR CRFLHDVGRY LETKPADLGP RCVLFETFGR CPYGVTCRFA 180
GAHLRPEGQN LVQEELAARG TQPPSIRNGL DKALQQQLRK REVRFERAEQ ALRRFSQGPT 240
PAAAVPEGTA AEGAPRQENC GAQQVPAGPG TSTPPSSPVR TCGPLTDEDV VRLRPCEKKR 300
LDIRGKLYLA PLTTCGNLPF RRICKRFGAD VTCGEMAVCT NLLQGQMSEW ALLKRHQCED 360
IFGVQLEGAF PDTMTKCAEL LSRTVEVDFV DINVGCPIDL VYKKGGGCAL MNRSTKFQQI 420
VRGMNQVLDV PLTVKIRTGV QERVNLAHRL LPELRDWGVA LVTLHGRSRE QRYTKLADWQ 480
YIEECVQAAS PMPLFGNGDI LSFEDANRAM QTGVTGIMIA RGALLKPWLF TEIKEQRHWD 540
ISSSERLDIL RDFTNYGLEH WGSDTQGVEK TRRFLLEWLS FLCRYVPVGL LERLPQRINE 600
RPPYYLGRDY LETLMASQKA ADWIRISEML LGPVPPSFAF LPKHKANAYK 650 
Gene Ontology
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0017150; F:tRNA dihydrouridine synthase activity; IEA:InterPro.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0002943; P:tRNA dihydrouridine synthesis; IEA:GOC. 
Interpro
 IPR013785; Aldolase_TIM.
 IPR001269; tRNA_hU_synthase.
 IPR018517; tRNA_hU_synthase_CS.
 IPR000571; Znf_CCCH. 
Pfam
 PF01207; Dus 
SMART
  
PROSITE
 PS01136; UPF0034
 PS50103; ZF_C3H1 
PRINTS