CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-038575
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Afadin 
Protein Synonyms/Alias
  
Gene Name
 Mllt4 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
383GSALPPEKLPYLVELubiquitination[1]
1699QRNASYLKTQVLSPDubiquitination[1]
1788QGQDVSDKVKASRKLacetylation[2, 3, 4]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1805 AA 
Protein Sequence
MSAGGRDEER RKLADIIHHW NANRLDLFEI SQPTEDLEFH GVMRFYFQDK AAGNFATKCI 60
RVSSTATTQD VIETLAEKFR PDMRMLSSPK YSLYEVHVSG ERRLDIDEKP LVVQLNWNKD 120
DREGRFVLKN ENDAIPAKKA QSNGPEKQEK EGVIQNFKRT LSKKEKKEKK KKEKEALRQA 180
SDKEERPSQG DDSENSRLAA EVYKDMPETS FTRTISNPEV VMKRRRQQKL EKRMQEFRSS 240
DGRPDSGGTL RIYADSLKPN IPYKTILLST TDTADFAVAE SLEKYGLEKE NPKDYCIARV 300
MLPPGAQHSD ERGAKEIILD DDECPLQIFR EWPSDKGILV FQLKRRPPDY IPKKMKKHVE 360
GKSLKGKDRA DGSGYGSALP PEKLPYLVEL SPDGSDSRDK PKLYRLQLSV TEVGTEKFDD 420
NSIQLFGPGI QPHHCDLTNM DGVVTVTPRS MDAETYVDGQ RISETTMLQS GMRLQFGTSH 480
VFKFVDPIQD HVLSKRSVDG GLMVKGPRHK PGAVQETTFE LGGDVHSGTA LPASRSTTRL 540
DSDRVSSASS TAERGMVKPM IRLDQEQEYR RRENRTQDAT GPELILPASI EFRESSEDSF 600
LSAIINYTNS STVHFKLSPT YVLYMACRYV LSSQHRPDIS PTERTHKAIA VVNKMVSMME 660
GVIQKQKNIA GALAFWMANA SELLNFIKQD RDLSRITLDA QDVLAHLVQM AFKYLVHCLQ 720
SELNNYMPAF LDDPEENSLQ RPKIDDVLHT LTGAMSLLRR CRVNAALTIQ LFSQLFHFIN 780
MWLFNRLVTD PDSGLCSHYW GAIIRQQLGH IEAWAEKQGL ELAADCHLSR IVQATTLLTM 840
DKYVPDDIPN INSTCFKLNS LQLQALLQNY HCAPDEPFIP TDLIENVVAV AENTADELAR 900
SDGRDVQLEE DPDLQLPFLL PEDGYSCDVV RNIPNGLQEF LDPLCQRGFC RLVPHTRSPG 960
TWTIYFEGAD YESHLMRENA ELATPLRKEP EIITVTLKKQ NGMGLSIVAA KGAGQDKLGI 1020
YVKSVVKGGA ADVDGRLAAG DQLLSVDGRS LVGLSQERAA ELMTRTSSVV TLEVAKQGAI 1080
YHGLATLLNQ PSPMMQRISD RRGSGKPRPK SEGFELYNNS AQNGSPESPQ MPWTEYSEPK 1140
KLPGDDRLMK NRADHRSSPN VANQPPSPGG KGPYTSGTAA KITSVSTGNL CTEEQSPPPR 1200
PEAYPIPTQT YTREYFTFPA SKSQDRMAPP QSQWPNYEEK PHVHTESNHS SIAIQRVTRS 1260
QEELREEKVY QLERHRVEAG MDRKCDSDMW INQSSSVESS TSSQEHLNHS SKSVTPASTL 1320
TKSGPGRWKT PAAVLPTPVA VSQPIRTDLP PPPPPPPVHY TSEFDGIPMD LPLPPPPANQ 1380
AGPQSAQVAA AEWKKREEHQ RWYEKEKARL EEERERKRRE QERKLGQMRS QTLNPASFSP 1440
LATQAKPEKP STLQRPQETV IRELQPQQQP RTIERKDLQY ITISKEELSS GDSLSPDPWK 1500
RDAREKLEKQ QQMHIVDMLS KEIHELQNKV DRTAEESDRL RKLMLEWQFQ KRLQESKQKD 1560
EDDDEEEDDD VDTMLIMQRL EAERRARMQD EERRRQQQLE EMRKREAEDR VRQEEDGRHQ 1620
EEERVKRDAE EKRRQEEGYY SRLEAERRRQ HEEAARRLLE PEEPGLSRPP LPRDYEPPSL 1680
SSAPCAPPPP PQRNASYLKT QVLSPDSLFT AKFVAYDEEE EDYGPAGPNS YSGSAGTAVG 1740
AYDAPREARE KLTRSQDADL PGSSGAPENL TFKERQRLFS QGQDVSDKVK ASRKLTELEN 1800
ELNTK 1805 
Gene Ontology
 GO:0045177; C:apical part of cell; IDA:MGI.
 GO:0005913; C:cell-cell adherens junction; IDA:MGI.
 GO:0005737; C:cytoplasm; IDA:MGI.
 GO:0007165; P:signal transduction; IEA:InterPro. 
Interpro
 IPR018444; Dil_domain.
 IPR002710; Dilute.
 IPR000253; FHA_dom.
 IPR001478; PDZ.
 IPR000159; Ras-assoc.
 IPR008984; SMAD_FHA_domain. 
Pfam
 PF01843; DIL
 PF00498; FHA
 PF00595; PDZ
 PF00788; RA 
SMART
 SM00240; FHA
 SM00228; PDZ
 SM00314; RA 
PROSITE
 PS51126; DILUTE
 PS50106; PDZ
 PS50200; RA 
PRINTS