UniProt Accession

 EDF1_MOUSE; Q9JMG1; Q99NE6 

Genbank Protein ID

 AB030185; AJ309569; AK002345; AK003557; AK010191; BC023472 

Genbank Nucleotide ID

 BAA92749.1; CAC32040.1; BAB22026.1; BAB22854.1; BAB26758.1; AAH23472.1 

Protein Name

 Endothelial differentiation-related factor 1 

Protein Synonyms/Alias

 EDF-1; Multiprotein-bridging factor 1; MBF1 

Gene Name

 Edf1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
25	TAAQAKSKQAILAAQ	acetylation	[1]
25	TAAQAKSKQAILAAQ	ubiquitination	[2]
79	RVTLEVGKVIQRGRQ	ubiquitination	[2]
93	QSKGLTQKDLATKIN	ubiquitination	[2]
98	TQKDLATKINEKPQV	ubiquitination	[2]
143	DIGKPIEKGPKAK**	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. Might function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism (By similarity). 

Sequence Annotation

 DOMAIN 81 135 HTH cro/C1-type.
 DNA_BIND 92 111 H-T-H motif (Potential).
 REGION 37 113 Interaction with NR5A2, PPARG and NR1H3
 REGION 69 108 Interaction with TBP and NR5A1 (By
 MOTIF 81 88 IQ motif (By similarity).
 MOD_RES 2 2 N-acetylalanine (By similarity).  

Keyword

 Acetylation; Activator; Calmodulin-binding; Complete proteome; Cytoplasm; Developmental protein; Differentiation; DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 148 AA 

Protein Sequence

Gene Ontology

 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005669; C:transcription factor TFIID complex; IEA:Compara.
 GO:0043565; F:sequence-specific DNA binding; IEA:InterPro.
 GO:0003713; F:transcription coactivator activity; IEA:Compara.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0043388; P:positive regulation of DNA binding; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR001387; HTH.
 IPR010982; Lambda_DNA-bd_dom.
 IPR013729; MBF1_N. 

Pfam

 PF01381; HTH_3
 PF08523; MBF1 

SMART

 SM00530; HTH_XRE 

PROSITE

 PS50943; HTH_CROC1 

PRINTS