CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023972
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ancient ubiquitous protein 1 
Protein Synonyms/Alias
  
Gene Name
 AUP1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
209GELVESLKRFCASTRubiquitination[1, 2, 3, 4, 5, 6, 7, 8]
316RVQQLVAKELGQTGTubiquitination[1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12]
330TRLTPADKAEHMKRQubiquitination[1, 5, 7, 8, 12]
335ADKAEHMKRQRHPRLubiquitination[2, 5]
425LPTASASKFPSSGPVubiquitination[1, 2, 3, 5, 6, 7, 8, 10, 12]
443PTALTFAKSSWARQEubiquitination[1, 2, 3, 5, 6, 7, 8, 12]
456QESLQERKQALYEYAubiquitination[5, 12]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [10] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [11] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [12] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965
Functional Description
 May play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. 
Sequence Annotation
 DOMAIN 362 404 CUE.
 MOD_RES 354 354 Phosphoserine.
 MOD_RES 358 358 Phosphoserine.
 MOD_RES 429 429 Phosphoserine.
 MOD_RES 433 433 Phosphothreonine.  
Keyword
 3D-structure; Alternative splicing; Complete proteome; Endoplasmic reticulum; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 476 AA 
Protein Sequence
MELPSGPGPE RLFDSHRLPG DCFLLLVLLL YAPVGFCLLV LRLFLGIHVF LVSCALPDSV 60
LRRFVVRTMC AVLGLVARQE DSGLRDHSVR VLISNHVTPF DHNIVNLLTT CSTVSESEAE 120
SATGRFPGAQ LKAPLSPLAF RMEDTEALPL TPILYPTCQF FFFIFLNIFL LAFSSPGSQP 180
LLNSPPSFVC WSRGFMEMNG RGELVESLKR FCASTRLPPT PLLLFPEEEA TNGREGLLRF 240
SSWPFSIQDV VQPLTLQVQR PLVSVTVSDA SWVSELLWSL FVPFTVYQVR WLRPVHRQLG 300
EANEEFALRV QQLVAKELGQ TGTRLTPADK AEHMKRQRHP RLRPQSAQSS FPPSPGPSPD 360
VQLATLAQRV KEVLPHVPLG VIQRDLAKTG CVDLTITNLL EGAVAFMPED ITKGTQSLPT 420
ASASKFPSSG PVTPQPTALT FAKSSWARQE SLQERKQALY EYARRRFTER RAQEAD 476 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:HPA. 
Interpro
 IPR003892; CUE. 
Pfam
 PF02845; CUE 
SMART
 SM00546; CUE 
PROSITE
 PS51140; CUE 
PRINTS