CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007898
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Spindle pole body assembly component MPS3 
Protein Synonyms/Alias
 98 kDa nuclear envelope protein; Monopolar spindle protein 3 
Gene Name
 MPS3 
Gene Synonyms/Alias
 NEP98; YJL019W; J1310; J1315; YJL018W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
147LESSGEAKKLKWRTYacetylation[1]
148ESSGEAKKLKWRTYIacetylation[1]
150SGEAKKLKWRTYIFYacetylation[1]
557KRDRNYIKIAKFEYRacetylation[2]
Reference
 [1] Acetylation of the SUN protein Mps3 by Eco1 regulates its function in nuclear organization.
 Ghosh S, Gardner JM, Smoyer CJ, Friederichs JM, Unruh JR, Slaughter BD, Alexander R, Chisholm RD, Lee KK, Workman JL, Jaspersen SL.
 Mol Biol Cell. 2012 Jul;23(13):2546-59. [PMID: 22593213]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Required for the first step of spindle pole body duplication in G1. Essential for nuclear division and fusion. Functions in sister chromatid cohesion establishment. Connects the spindle pole body with the nuclear envelope through its interaction with MPS2 and mediates meiotic bouquet formation and rapid chromosome movements in meiotic prophase. Functions as an integral membrane anchor for telomeres and is a nuclear receptor for the SIR4 pathway of telomere tethering and gene inactivation. recruits double-strand breaks (DSBs) to the nuclear periphery for chromosome healing. 
Sequence Annotation
 DOMAIN 427 616 SUN.
 NP_BIND 187 194 ATP (Potential).
 REGION 171 682 Interaction with CDC31.
 REGION 431 650 Interaction with JEM1.  
Keyword
 ATP-binding; Cell cycle; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Membrane; Nucleotide-binding; Nucleus; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 682 AA 
Protein Sequence
MNNSNEHRRE EAGAANEQMP YNKAVKSAYA DVLKDKMNRE QEISLRAIKK GIYTDGGETD 60
NYDMDKENDS AYEMFKKNLD FPLDQHNDDD DDDPYIEDNG QETDGYSDED YTDEADKSFI 120
EDSDSDSYDL ESNSDFEENL ESSGEAKKLK WRTYIFYGGL FFVFYFFGSF LMTTVKNNDL 180
ESHSSGATSS PGKSFSNLQK QVNHLYSELS KRDEKHSSEL DKTVKIIVSQ FEKNIKRLLP 240
SNLVNFENDI NSLTKQVETI STSMSELQRR NHKFTVENVT QWQDQLVKQL DTHLPQEIPV 300
VINNSSSLLI IPELHNYLSA LISDVIESPG IGTAGSAESR WEYDLNRYVK EILSNELQYI 360
DKDYFIQEMN RRLQSNKQEI WEEITNRLET QQQQQQQQVQ QDYSNVPQQY SSILMKRLIH 420
QIYNSNQHQW EDDLDFATYV QGTKLLNHLT SPTWRQGSGV QPIELLTDSK QSSSTYWQCE 480
NEPGCSWAIR FKTPLYLTKI SYMHGRFTNN LHIMNSAPRL ISLYVKLSQT KEIKALQTLA 540
NQYGFGQHHK RDRNYIKIAK FEYRLTDSRI RQQMYLPPWF IQLKPLVRSI VFQVDENYGN 600
KKFISLRKFI INGVTPQDLQ IIENNEFPVL LGDTPEYGVT QNTDEGKRKV LLSKPPYASS 660
STSTKFHPAS NVPSFGQDEL DQ 682 
Gene Ontology
 GO:0005825; C:half bridge of spindle pole body; IDA:SGD.
 GO:0016021; C:integral to membrane; IDA:SGD.
 GO:0000784; C:nuclear chromosome, telomeric region; IDA:SGD.
 GO:0005635; C:nuclear envelope; IDA:SGD.
 GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
 GO:0034399; C:nuclear periphery; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0006348; P:chromatin silencing at telomere; IMP:SGD.
 GO:0034087; P:establishment of mitotic sister chromatid cohesion; IMP:SGD.
 GO:0045141; P:meiotic telomere clustering; IMP:SGD.
 GO:0000743; P:nuclear migration involved in conjugation with cellular fusion; IMP:SGD.
 GO:0007103; P:spindle pole body duplication associated with nuclear envelope; IMP:SGD.
 GO:0007129; P:synapsis; IMP:SGD.
 GO:0034398; P:telomere tethering at nuclear periphery; IMP:SGD. 
Interpro
 IPR012919; Sad1_UNC_C. 
Pfam
  
SMART
  
PROSITE
 PS51469; SUN 
PRINTS