CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009589
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 40S ribosomal protein S25 
Protein Synonyms/Alias
  
Gene Name
 Rps25 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
18DAGKSAKKDKDPVNKacetylation[1]
20GKSAKKDKDPVNKSGacetylation[1]
43SKGKVRDKLNNLVLFacetylation[2, 3]
43SKGKVRDKLNNLVLFubiquitination[4]
52NNLVLFDKATYDKLCacetylation[1, 2, 3, 5, 6, 7]
52NNLVLFDKATYDKLCsuccinylation[3]
52NNLVLFDKATYDKLCubiquitination[4]
57FDKATYDKLCKEVPNacetylation[1, 2, 3, 7]
60ATYDKLCKEVPNYKLacetylation[1, 7]
66CKEVPNYKLITPAVVacetylation[1, 2, 3, 6, 7, 8]
66CKEVPNYKLITPAVVubiquitination[4]
94ALQELLSKGLIKLVSacetylation[1, 2, 3]
94ALQELLSKGLIKLVSsuccinylation[3]
98LLSKGLIKLVSKHRAacetylation[1]
Reference
 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [7] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [8] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758
Functional Description
  
Sequence Annotation
 MOD_RES 52 52 N6-acetyllysine (By similarity).
 MOD_RES 60 60 N6-acetyllysine (By similarity).
 MOD_RES 66 66 N6-acetyllysine (By similarity).
 MOD_RES 94 94 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Complete proteome; Reference proteome; Ribonucleoprotein; Ribosomal protein. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 125 AA 
Protein Sequence
MPPKDDKKKK DAGKSAKKDK DPVNKSGGKA KKKKWSKGKV RDKLNNLVLF DKATYDKLCK 60
EVPNYKLITP AVVSERLKIR GSLARAALQE LLSKGLIKLV SKHRAQVIYT RNTKGGDAPA 120
AGEDA 125 
Gene Ontology
 GO:0022627; C:cytosolic small ribosomal subunit; IEA:Compara.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0003735; F:structural constituent of ribosome; IEA:Compara.
 GO:0000028; P:ribosomal small subunit assembly; IEA:Compara. 
Interpro
 IPR004977; Ribosomal_S25. 
Pfam
 PF03297; Ribosomal_S25 
SMART
  
PROSITE
  
PRINTS