UniProt Accession

 PURA2_MOUSE; P46664; Q9CQL9 

Genbank Protein ID

 L24554; AK004877; AK010263; AK028060; AK148420; AK170279 

Genbank Nucleotide ID

 AAA19727.1; BAB23635.1; BAB26805.1; BAC25730.1; BAE28543.1; BAE41682.1 

Protein Name

 Adenylosuccinate synthetase isozyme 2 

Protein Synonyms/Alias

 AMPSase 2; AdSS 2; Adenylosuccinate synthetase, acidic isozyme; Adenylosuccinate synthetase, liver isozyme; L-type adenylosuccinate synthetase; IMP--aspartate ligase 2 

Gene Name

 Adss 

Gene Synonyms/Alias

 Adss2 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
45	WGDEGKGKVVDLLAQ	acetylation	[1]
118	EKNVQKGKGLDGWEK	ubiquitination	[2]
173	IGPVYSSKAARSGLR	ubiquitination	[2]
196	DGFSERFKVLANQYK	ubiquitination	[2]
419	ISNARTFKELPVNAQ	acetylation	[1, 3]
419	ISNARTFKELPVNAQ	ubiquitination	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP. 

Sequence Annotation

 NP_BIND 39 45 GTP (By similarity).
 NP_BIND 67 69 GTP (By similarity).
 NP_BIND 362 364 GTP (By similarity).
 NP_BIND 444 447 GTP (By similarity).
 REGION 40 43 IMP binding (By similarity).
 REGION 65 68 IMP binding (By similarity).
 REGION 330 336 Substrate binding (By similarity).
 ACT_SITE 40 40 Proton acceptor (By similarity).
 ACT_SITE 68 68 Proton donor (By similarity).
 METAL 40 40 Magnesium (By similarity).
 METAL 67 67 Magnesium; via carbonyl oxygen (By
 BINDING 40 40 Substrate (By similarity).
 BINDING 162 162 IMP (By similarity).
 BINDING 176 176 IMP; shared with dimeric partner (By
 BINDING 255 255 IMP (By similarity).
 BINDING 270 270 IMP (By similarity).
 BINDING 334 334 IMP (By similarity).
 BINDING 336 336 GTP (By similarity).  

Keyword

 Complete proteome; Cytoplasm; GTP-binding; Ligase; Magnesium; Metal-binding; Nucleotide-binding; Purine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 456 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005886; C:plasma membrane; IEA:Compara.
 GO:0004019; F:adenylosuccinate synthase activity; IDA:MGI.
 GO:0005525; F:GTP binding; IEA:HAMAP.
 GO:0000287; F:magnesium ion binding; IEA:HAMAP.
 GO:0044208; P:'de novo' AMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006167; P:AMP biosynthetic process; IDA:MGI.
 GO:0006531; P:aspartate metabolic process; IEA:Compara.
 GO:0071257; P:cellular response to electrical stimulus; IEA:Compara.
 GO:0006184; P:GTP catabolic process; IEA:Compara.
 GO:0046040; P:IMP metabolic process; IEA:Compara.
 GO:0060359; P:response to ammonium ion; IEA:Compara.
 GO:0014074; P:response to purine-containing compound; IEA:Compara. 

Interpro

 IPR018220; Adenylosuccinate_synthase_AS.
 IPR001114; Adenylosuccinate_synthetase.
 IPR027529; AdSS_2_vert.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00709; Adenylsucc_synt 

SMART

 SM00788; Adenylsucc_synt 

PROSITE

 PS01266; ADENYLOSUCCIN_SYN_1
 PS00513; ADENYLOSUCCIN_SYN_2 

PRINTS