CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000469
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Circadian locomoter output cycles protein kaput 
Protein Synonyms/Alias
 hCLOCK; Class E basic helix-loop-helix protein 8; bHLHe8 
Gene Name
 CLOCK 
Gene Synonyms/Alias
 BHLHE8; KIAA0334 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31GLVEEDDKDKAKRVSacetylation[1]
41AKRVSRNKSEKKRRDacetylation[1]
85IDFLRKHKEITAQSDubiquitination[2]
186SLTPEYLKSKNQLEFubiquitination[2]
402LPETAADKSQDSGSDubiquitination[2]
418RINTVSLKEALERFDubiquitination[2]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 ARNTL/2-CLOCK heterodimers activate E-box element (5'- CACGTG-3') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. Has intrinsic histone acetyltransferase activity and this enzymatic function contributes to chromatin-remodeling events implicated in circadian control of gene expression (By similarity). Acetylates primarily histones H3 and H4 (By similarity). Acetylates also a non-histone substrate: ARNTL (By similarity). Plays a role in DNA damage response (DDR) signaling during the S phase. 
Sequence Annotation
 DOMAIN 34 84 bHLH.
 DOMAIN 107 177 PAS 1.
 DOMAIN 262 332 PAS 2.
 DOMAIN 336 379 PAC.
 REGION 514 564 Implicated in the circadian rhythmicity
 MOD_RES 408 408 Phosphoserine (By similarity).  
Keyword
 3D-structure; Activator; Acyltransferase; Biological rhythms; Chromosome; Complete proteome; Cytoplasm; DNA damage; DNA-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 846 AA 
Protein Sequence
MLFTVSCSKM SSIVDRDDSS IFDGLVEEDD KDKAKRVSRN KSEKKRRDQF NVLIKELGSM 60
LPGNARKMDK STVLQKSIDF LRKHKEITAQ SDASEIRQDW KPTFLSNEEF TQLMLEALDG 120
FFLAIMTDGS IIYVSESVTS LLEHLPSDLV DQSIFNFIPE GEHSEVYKIL STHLLESDSL 180
TPEYLKSKNQ LEFCCHMLRG TIDPKEPSTY EYVKFIGNFK SLNSVSSSAH NGFEGTIQRT 240
HRPSYEDRVC FVATVRLATP QFIKEMCTVE EPNEEFTSRH SLEWKFLFLD HRAPPIIGYL 300
PFEVLGTSGY DYYHVDDLEN LAKCHEHLMQ YGKGKSCYYR FLTKGQQWIW LQTHYYITYH 360
QWNSRPEFIV CTHTVVSYAE VRAERRRELG IEESLPETAA DKSQDSGSDN RINTVSLKEA 420
LERFDHSPTP SASSRSSRKS SHTAVSDPSS TPTKIPTDTS TPPRQHLPAH EKMVQRRSSF 480
SSQSINSQSV GSSLTQPVMS QATNLPIPQG MSQFQFSAQL GAMQHLKDQL EQRTRMIEAN 540
IHRQQEELRK IQEQLQMVHG QGLQMFLQQS NPGLNFGSVQ LSSGNSSNIQ QLAPINMQGQ 600
VVPTNQIQSG MNTGHIGTTQ HMIQQQTLQS TSTQSQQNVL SGHSQQTSLP SQTQSTLTAP 660
LYNTMVISQP AAGSMVQIPS SMPQNSTQSA AVTTFTQDRQ IRFSQGQQLV TKLVTAPVAC 720
GAVMVPSTML MGQVVTAYPT FATQQQQSQT LSVTQQQQQQ SSQEQQLTSV QQPSQAQLTQ 780
PPQQFLQTSR LLHGNPSTQL ILSAAFPLQQ STFPQSHHQQ HQSQQQQQLS RHRTDSLPDP 840
SKVQPQ 846 
Gene Ontology
 GO:0005694; C:chromosome; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005667; C:transcription factor complex; IPI:MGI.
 GO:0070888; F:E-box binding; IDA:BHF-UCL.
 GO:0004402; F:histone acetyltransferase activity; IEA:EC.
 GO:0000978; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0000982; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity; ISS:BHF-UCL.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; ISS:BHF-UCL.
 GO:0004871; F:signal transducer activity; IEA:InterPro.
 GO:0071479; P:cellular response to ionizing radiation; IDA:UniProtKB.
 GO:0032922; P:circadian regulation of gene expression; ISS:UniProtKB.
 GO:0000077; P:DNA damage checkpoint; IMP:UniProtKB.
 GO:0016573; P:histone acetylation; IEA:GOC.
 GO:0009648; P:photoperiodism; TAS:ProtInc.
 GO:0007165; P:signal transduction; TAS:ProtInc. 
Interpro
 IPR011598; bHLH_dom.
 IPR001067; Nuc_translocat.
 IPR001610; PAC.
 IPR000014; PAS.
 IPR013767; PAS_fold.
 IPR013655; PAS_fold_3. 
Pfam
 PF00010; HLH
 PF00989; PAS
 PF08447; PAS_3 
SMART
 SM00353; HLH
 SM00086; PAC
 SM00091; PAS 
PROSITE
 PS50888; BHLH
 PS50113; PAC
 PS50112; PAS 
PRINTS
 PR00785; NCTRNSLOCATR.