CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002464
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic translation initiation factor 4E 
Protein Synonyms/Alias
 eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein 
Gene Name
 CDC33 
Gene Synonyms/Alias
 TIF45; YOL139C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
9SVEEVSKKFEENVSVacetylation[1]
9SVEEVSKKFEENVSVubiquitination[2]
36DSAHFDVKHPLNTKWacetylation[1]
111WEDEANAKGGKWSFQubiquitination[2, 3]
114EANAKGGKWSFQLRGacetylation[1]
114EANAKGGKWSFQLRGubiquitination[2, 3]
162SIRKGGNKFALWTKSacetylation[1]
162SIRKGGNKFALWTKSubiquitination[2]
172LWTKSEDKEPLLRIGacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047
Functional Description
 Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. 
Sequence Annotation
 MOD_RES 2 2 Phosphoserine; by CK2.
 MOD_RES 15 15 Phosphoserine; by CK2.
 MOD_RES 22 22 Phosphothreonine.
 MOD_RES 28 28 Phosphoserine.
 MOD_RES 30 30 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome; RNA-binding; Translation regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 213 AA 
Protein Sequence
MSVEEVSKKF EENVSVDDTT ATPKTVLSDS AHFDVKHPLN TKWTLWYTKP AVDKSESWSD 60
LLRPVTSFQT VEEFWAIIQN IPEPHELPLK SDYHVFRNDV RPEWEDEANA KGGKWSFQLR 120
GKGADIDELW LRTLLAVIGE TIDEDDSQIN GVVLSIRKGG NKFALWTKSE DKEPLLRIGG 180
KFKQVLKLTD DGHLEFFPHS SANGRHPQPS ITL 213 
Gene Ontology
 GO:0010494; C:cytoplasmic stress granule; IDA:SGD.
 GO:0016281; C:eukaryotic translation initiation factor 4F complex; TAS:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0005840; C:ribosome; TAS:SGD.
 GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:SGD.
 GO:0003743; F:translation initiation factor activity; ISS:SGD.
 GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IDA:SGD.
 GO:0051726; P:regulation of cell cycle; IMP:SGD.
 GO:0006417; P:regulation of translation; IEA:UniProtKB-KW. 
Interpro
 IPR023398; TIF_eIF4e-like_dom.
 IPR001040; TIF_eIF_4E.
 IPR019770; TIF_eIF_4E_CS. 
Pfam
 PF01652; IF4E 
SMART
  
PROSITE
 PS00813; IF4E 
PRINTS