CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023870
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sorting and assembly machinery component 50 homolog 
Protein Synonyms/Alias
 Transformation-related gene 3 protein; TRG-3 
Gene Name
 SAMM50 
Gene Synonyms/Alias
 CGI-51; SAM50; TRG3 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
36VEVEPEAKQEILENKubiquitination[1]
59FDGLGRTKDDIIICEubiquitination[2, 3, 4, 5]
72CEIGDVFKAKNLIEVubiquitination[2, 4]
74IGDVFKAKNLIEVMRubiquitination[4]
148GSMVLGLKLPNLLGRubiquitination[2]
178SYGLSFFKPRPGNFEubiquitination[4, 5]
227WKTSHTVKWEGVWREubiquitination[4]
297GGDVSFIKEDFELQLubiquitination[1]
412LNYGEGPKAHIRKLAubiquitination[4]
417GPKAHIRKLAECIRWubiquitination[4]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 May be required for the assembly pathway of mitochondrial outer membrane proteins (By similarity). 
Sequence Annotation
  
Keyword
 Complete proteome; Cytoplasm; Membrane; Mitochondrion; Mitochondrion outer membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane beta strand. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 469 AA 
Protein Sequence
MGTVHARSLE PLPSSGPDFG GLGEEAEFVE VEPEAKQEIL ENKDVVVQHV HFDGLGRTKD 60
DIIICEIGDV FKAKNLIEVM RKSHEAREKL LRLGIFRQVD VLIDTCQGDD ALPNGLDVTF 120
EVTELRRLTG SYNTMVGNNE GSMVLGLKLP NLLGRAEKVT FQFSYGTKET SYGLSFFKPR 180
PGNFERNFSV NLYKVTGQFP WSSLRETDRG MSAEYSFPIW KTSHTVKWEG VWRELGCLSR 240
TASFAVRKES GHSLKSSLSH AMVIDSRNSS ILPRRGALLK VNQELAGYTG GDVSFIKEDF 300
ELQLNKQLIF DSVFSASFWG GMLVPIGDKP SSIADRFYLG GPTSIRGFSM HSIGPQSEGD 360
YLGGEAYWAG GLHLYTPLPF RPGQGGFGEL FRTHFFLNAG NLCNLNYGEG PKAHIRKLAE 420
CIRWSYGAGI VLRLGNIARL ELNYCVPMGV QTGDRICDGV QFGAGIRFL 469 
Gene Ontology
 GO:0016021; C:integral to membrane; IDA:BHF-UCL.
 GO:0005743; C:mitochondrial inner membrane; IEA:Compara.
 GO:0001401; C:mitochondrial sorting and assembly machinery complex; IMP:BHF-UCL.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0045040; P:protein import into mitochondrial outer membrane; IDA:BHF-UCL. 
Interpro
 IPR000184; Bac_surfAg_D15. 
Pfam
 PF01103; Bac_surface_Ag 
SMART
  
PROSITE
  
PRINTS