CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024471
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Puromycin-sensitive aminopeptidase-like protein 
Protein Synonyms/Alias
  
Gene Name
 NPEPPSL1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
48GLAAMPEKRPFERLPacetylation[1]
68INCSLCLKPDLLDFTubiquitination[2]
155FVGELNDKMKGFYRSubiquitination[3, 4, 5, 6]
157GELNDKMKGFYRSKYubiquitination[5]
208DISLVVPKDRVALSNubiquitination[3]
222NMNVIDRKPYPDDENubiquitination[4, 5]
234DENLVEVKFARTPVTubiquitination[2, 3, 4, 5, 7]
261DFVETRSKDGVCVCVubiquitination[5]
274CVYTPVGKAEQGKFAubiquitination[2, 5]
279VGKAEQGKFALEVAAubiquitination[5]
287FALEVAAKTLPFYKDubiquitination[3, 4]
293AKTLPFYKDYFNVPYubiquitination[2, 3, 4, 5, 7]
440FDAISYSKGASVIRMubiquitination[2, 4, 7]
455LHDYIGDKDFKKGMNacetylation[8]
455LHDYIGDKDFKKGMNubiquitination[3, 5]
459IGDKDFKKGMNMYLTubiquitination[5]
467GMNMYLTKFQQKNAAubiquitination[3]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377
Functional Description
 Aminopeptidase with broad substrate specificity to several peptides (By similarity). 
Sequence Annotation
 REGION 316 320 Substrate binding (By similarity).
 ACT_SITE 353 353 Proton acceptor (By similarity).
 METAL 352 352 Zinc; catalytic (By similarity).
 METAL 356 356 Zinc; catalytic (By similarity).
 METAL 375 375 Zinc; catalytic (By similarity).
 BINDING 180 180 Substrate (By similarity).  
Keyword
 Alternative splicing; Aminopeptidase; Complete proteome; Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome; Zinc. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 478 AA 
Protein Sequence
MWLAAAAPSL ARRLLFLGPP PPPLLLLVFS RSSRRRLHSL GLAAMPEKRP FERLPADVSP 60
INCSLCLKPD LLDFTFEGKL EAAAQVRQAT NQIVMNCADI DIITASYAPE GDEEIHATGF 120
NYQNEDEKVT LSFPSTLQTG TGTLKIDFVG ELNDKMKGFY RSKYTTPSGE VRYAAVTQFE 180
ATDARRAFPC WDERAIKATF DISLVVPKDR VALSNMNVID RKPYPDDENL VEVKFARTPV 240
TSTYLVAFVV GEYDFVETRS KDGVCVCVYT PVGKAEQGKF ALEVAAKTLP FYKDYFNVPY 300
PLPKIDLIAI ADFAAGAMEN WDLVTYRETA LLIDPKNSCS SSRQWVALVV GHELAHQWFG 360
NLVTMEWWTH LRLNEGFASW IEYLCVDHCF PEYDIWTQFV SADYTRAQEL DALDNSHPIE 420
VSVGHPSEVD EIFDAISYSK GASVIRMLHD YIGDKDFKKG MNMYLTKFQQ KNAAAGNL 478 
Gene Ontology
 GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
 GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW. 
Interpro
 IPR001930; Peptidase_M1.
 IPR014782; Peptidase_M1_N.
 IPR015568; Peptidase_M1_puromycin-sens. 
Pfam
 PF01433; Peptidase_M1 
SMART
  
PROSITE
 PS00142; ZINC_PROTEASE 
PRINTS
 PR00756; ALADIPTASE.