UniProt Accession

 RBM22_MOUSE; Q8BHS3; Q3TJB0; Q9CXA0 

Genbank Protein ID

 AK018452; AK045116; AK150271; AK151758; AK152252; AK166600; AK167509; AK168919; BC080205 

Genbank Nucleotide ID

 BAB31220.1; BAC32229.1; BAE29427.1; BAE30665.1; BAE31073.1; BAE38885.1; BAE39585.1; BAE40732.1; AAH80205.1 

Protein Name

 Pre-mRNA-splicing factor RBM22 

Protein Synonyms/Alias

 RNA-binding motif protein 22 

Gene Name

 Rbm22 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
212	INDPVADKLLKRAST	acetylation	[1, 2, 3]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 Involved in the first step of pre-mRNA splicing. Binds directly to the internal stem-loop (ISL) domain of the U6 snRNA and to the pre-mRNA intron near the 5' splice site during the activation and catalytic phases of the spliceosome cycle. Involved in both translocations of the nuclear SLU7 to the cytoplasm and the cytosolic calcium-binding protein PDCD6 to the nucleus upon cellular stress responses (By similarity). 

Sequence Annotation

 DOMAIN 232 305 RRM.
 ZN_FING 159 186 C3H1-type.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 212 212 N6-acetyllysine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Metal-binding; mRNA processing; mRNA splicing; Nucleus; Reference proteome; RNA-binding; Spliceosome; Transport; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 420 AA 

Protein Sequence

Gene Ontology

 GO:0071013; C:catalytic step 2 spliceosome; IEA:Compara.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0005487; F:nucleocytoplasmic transporter activity; ISS:UniProtKB.
 GO:0000166; F:nucleotide binding; IEA:InterPro.
 GO:0036002; F:pre-mRNA binding; ISS:UniProtKB.
 GO:0017069; F:snRNA binding; ISS:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0033554; P:cellular response to stress; ISS:UniProtKB.
 GO:0045292; P:mRNA cis splicing, via spliceosome; ISS:UniProtKB.
 GO:0090316; P:positive regulation of intracellular protein transport; ISS:UniProtKB.
 GO:0033120; P:positive regulation of RNA splicing; ISS:UniProtKB.
 GO:0000060; P:protein import into nucleus, translocation; IEA:Compara. 

Interpro

 IPR012677; Nucleotide-bd_a/b_plait.
 IPR000504; RRM_dom.
 IPR000571; Znf_CCCH. 

Pfam

 PF00076; RRM_1 

SMART

 SM00360; RRM
 SM00356; ZnF_C3H1 

PROSITE

 PS50102; RRM
 PS50103; ZF_C3H1 

PRINTS